| PurposeThis study aimed to test the expression of CD147 mRNA and its protein in of human bladder transitional cell carcinoma (BTCC) tissue and normal bladder mucous membrane tissue, and to investigate its relationships with clinical and pathological features including pathological grade and clinical stage of BTCC. This study has contributed to a better understanding the biological behavior of BTCC. It has also laid a solid foundation for future study of the interference of CD147RNA at the cellular level to find a new target for treatment of human BTCC.Materials and methodsIn this experiment, 67 BTCC tissues removed in open surgery were collected to form the experimental group. The comparison group consists of 12 normal bladder tissues obtained from patients with benign prostatic hyperplasia and bladder calculus during open surgery. All specimen were sliced and their CD147mRNA and protein expression were examined using nucleic acid molecule in situ hybridization and immunohistochemical methods. According to the number of positive cells, they were divided into: proportion of negative (-), positive (+), strong positive (+ +). Theχ~2 test, Fisher precisely the probability method and R x C column contingency table were adopted for correlation analysis, with p﹤0.05 indicating the differences were of statistical significance.Results1. The protein expression of CD147 of normal bladder and BTCC tissues, and their relationships with part of features of the clinical and pathological of BTCC. The expression of CD147 in the 12 cases of normal bladder tissue was negative. However, in 67 cases of BTCC tissues, 46 cases (68.7%) had CD147 protein expression, of which 11 cases (16.4%) showed strong positive expression. CD147 protein expression was mainly found in tumor cell membranes and cytoplasm. There was no obvious expression in tumor interstitial. The 67 patients were grouped according to the pathological grade and clinical stage of BTCC. There was no significant difference in the group structure in terms of age and sex (p≥0.05). CD147 protein expression was correlated with pathologic stage, which means when pathologic stage increased, CD147 protein expression was higher (r = 0.477, p < 0.001). However, he correlation of superficial cancer (Ta ~T1) and invasive carcinoma (T2 ~ T4) was not statistically significant (r = 0.142, p = 0.253).2.The CD147mRNA expression of normal bladder and BTCC tissues, and their relationships of part of features of the clinical and pathological of BTCC. The CD147mRNA expression in the 12 cases of normal bladder tissue was negative. However, in 67 cases of BTCC tissues, 40 cases (59.7%) had CD147mRNA expression, of which 4 patients (6%) showed strong positive expression. CD147mRNA expression was mainly found in the cytoplasm of the tumor. There was no obvious expression in tumor stroma. The correlations between CD147mRNA expression and the pathological grade and clinical stage of BTCC were not statistically significant (r=0.222, p=0.071; r=0.048, p=0.698).3. The correlation between CD147mRNA and CD147 protein expression in BTCC. CD147mRNA and CD147 protein of BTCC almost expressed at the same time, and their expression in BTCC were positively correlated (r=0.285, p=0.019).Conclusion1. There were CD147mRNA and CD147 protein expression in BTCC, but these expressions were not found in normal bladder mucous membrane.2. CD147 protein expression was mainly found in tumor cell membranes and cytoplasm. There was no obvious expression in tumor interstitial. The expression was not significantly correlated with age and sex of the patients, but correlated with the pathological grade. When the pathological grade increased, CD147 protein expression increased. The CD147 protein expression, however, was not significantly correlated with superficial cancer and invasive cancer.3. CD147mRNA expression was mainly found in the cytoplasm of the tumor. There was no obviously expression in interstitial tumor. The CD147mRNA expression was not significantly correlated with the clinical stage and pathological grade of BTCC.4. There were CD147mRNA and CD147 protein expression in BTCC. CD147mRNA and CD147 protein expression were positively correlated. CD147 may become an importance indicators of the invasiveness of BTCC.5. CD147 as a versatile molecule of tumor cell surface can regulate the key steps in tumor invasion and metastasis. Therefore, blockage of the CD147expression or its function may become a new target for treatment of BTCC.
|
PDF Full Text Request