Intrarterial Administration Of Naked Plasmid Encoding Human Hpatocyte Growth Factor In Canines, An Experiment Study

Objective: To evaluate the efficiency of gene transfer and expression by intrarterial injecting naked plasmid encoding human hepatocyte growth factor (HGF).Methods: Six canines were divided into study and control group. Naked plasmid encoding HGF was administrated in the artery of the left kidney in the four canines (study group), while control empty vector was injected in an identical manner in the other two (control group). Groups of canines were sacrificed at day 3 after injection procedure, respectively, and the left kidneys were removed for ELISA.Results: Human HGF protein was detected in the tissue of the left kidneys of the study group, but no detectable human HGF protein was found in left kidneys of control group.Conclusion: Intrarterial administration of naked plasmid encoding human HGF can be an efficient method to express human HGF protein locally.