Study On Anti-figure And Anti-hypoxia Effect Of Honeybee Pupae

The anti-fatigue and the anti-hypoxia function of honeybee pupae were studied in this text. Firstly proceeded to its measures-effect experiment, and on the basis of this experiment, adopted the different polarity melting agent to extract the powder of honeybee pupae and obtain different extraction. Further the animal experiment, compared the effect of different extraction on anti-fatigue and anti-hypoxia function, aim to know the distribution of components developing anti-fatigue and anti-hypoxia effect. Finally with the extraction developing biggest effect as experiment stuff, proceeded to the animal physiology and biochemistry experiment, trying to explain the anti-fatigue mechanism of honeybee pupae or its extraction from the angles of physiology and biochemistry.1. In the measure-effect experiment, with Kunming grows small rat as experiment object, designed 5 dose measures and 1 normal control, gave the honeybee pupae to experiment rats and kept on experiment a month of period above. In the period of experiment observed the feed consuming rate of experiment rats on the 7th, 14th, 21th day and body weight changes on the 9th, 18th, 27th day after giving honeybee pupae. After 30 day's treat with honeybee pupae, all animal proceeded to the swimming experiments and climbing pole experiment, observed the influence of honeybee pupae on anti-fatigue ability. After the Sport experiment, kept on giving honeybee to rat for 5 day as before, and then proceeded to normal-press anti-hypoxia experiments, observed the influence of honeybee pupae on anti-hypoxia ability. After, continued giving the honeybee pupae to experiment rats for 5 day and then proceeded to acute cerebral ischemia experiment, observed the survival time of rats. While proceeded to the acute cerebral ischemia experiment, immediately separated out the rat heart, liver, kidney and spleen and computed their organ index number.Result indicates: The honeybee pupae can increase the feed consuming rate and body weight of the rat. compared with normal control group , the effect showed no difference, and dose groups of 7g/(kgbw.d) and 10g/(kgbw.d) have significant difference on swimming time and climbing pole time(.P<0.05, P<0.01) in exercise endurance experiment and on normal-press anti-hypoxia time (P<0.05, P<0.01), which showed out their dependence on dose of honeybee pupae, but have no influence on acute cerebral ischemia hypoxia death time and the organ index number of the rat in experiment(P >0.05).2.Used the petroleum ether, ethyl acetate, ether and water in turns to extract honeybee pupae powder and obtained Ea( the extraction from petroleum ether), Ee( the extraction from ethyl acetate), Eet(the extraction from ether), Ew(the extraction from water) and Dr(drain). Regarded above five...