Effect Of Epidermal Growth Factor In Tissue Expansion And E-cadherin Expression In Expanded Tissues

Objective To investigate effect and possibility of local application of EGF gel during continuous tissue expansion (CTE) and expression of E-cadherin in tissue expansion.Methods (1)To get different concentration gel , rhEGF gel with 2% Azone was diffused with distilled water .The mixture was under RT or 4℃ for 48h .The mixture was used on the surface of medial forearm skin . The skin reactions were evaluated 24h later. (2)The liquid was injected into expander under 40, 60, 80cmH₂O for once a day or every two day. the reaction of patients and injection volume were investigated under different pressure. (3)The experiment was divided into 3 groups with self-control: Group 1: 5 μ g/g rhEGF gel and blank control; Group 2: 10μ g/g rhEGF gel and blank control; Group 3: 5μg/g rhEGF gel and 10μ g/g rhEGF gel. Expansion rate and injection valume were counted while gels were used on the surface of skin three times one day during expansion. The histology change of skins were observed by HE dyeing. (4)E-cadherin distribution and expression were measured with S-ABC, the result was used for image analysing. Results: (1) 10μg/g and 5μg/g rhEGF gels with 2% Azone, which were shining ivory and homogeneous, can be stable and had no skin reaction. 2.5μg/g rhEGF gel was just like water and cannot adhere to the skin, so it is...