Fibroblast Growth Factor Enhances Bone Formation Induced By Bone Morphogenetic Protein In Dog's Trachea

Tracheal transplantation is one of important anticipated events in tracheal surgery, being regarded as an ideal method for reconstruction of tracheal defect after extensive resection. The research on tracheal trsplantation progressed slowly in last century because of the subsequent severe stenosis and obliteration of the tracheal grafts. The major causes consisted of ischemia and obliteration of the tracheal grafts, which result in cartilaginous necrosis, chondrolysis and resorption. Although different processes of airway collapse have been found in tracheal grafts treated by different methods, few suitable methods can be applied to solved the problem essentially up to date. So it is necessary to find a new method to prevent the tracheal grafts from stenosis. Bone morphogenetic proteins (BMPs) are a group of proteins which can induce new bone and/or cartilage formation heterotopically. Basic fibroblast growth factor (bFGF) is a powerful micrangium proliferative stimulator, which can stimulate chondroblasts and chondrocytes multiplication, but also enhance the function of BMP. In this study BMP and bFGF were used to meet the problem.Objective: Aim to induce cartilage regeneration in trachea by BMP and bFGF, and ultimately form a new elastic stent for trachea grafts to protect the airway from collapse.Methods: Canine tracheal cartilage defect models were made surgically and then BMP and bFGF were implanted into the defects to induce cartilage regeneration.