Expression And Clinical Significance Of Vascular Endothelial Growth Factor And Platelet Derived Growth Factor In Degenerated Intervertebral Disc

PrefaceDegeneration of intervertebral disc is a term to describe the loss of normal structure of intervertebral disc accompanied with the progressive fibrosis. The manifestations are gelatinous disappearance of pulpiform nucleus, rough lamellar bone of annulus fibrosis and progressive fibrosis. Finally, fissure and senile chromatosis ensure. The degeneration of intervertebral disc is associated with the release of some cytokines and the changes of enzymes activity in the intervertebral disc, vascular endothelial growth factor and platelet derived growth factor are two important cytokines with special relationship. It is not clear how the cytokines afiFect the degeneration of the intervertebral disc. The amount changes of vascular endothelial growth factor and platelet derived growth factor in the intervertebral disc and their relationship were discussed in this study.Materials and Methods1 basic research1.1 animals and grouping:50 male Wistar rats were selected (10 each group of 1,3,6, 12,18 months old) and divided into 5 groups by monthage.1. 2 specimen selection: kill the rats by pneumothoras and get the vertebrae of certain segment, fix the specimen in 10% formaldehyde and 2. 5% glutaral-dehyde(GA).1. 3 method of test:1.3.1 survey the histological structure of the rat intervertebral disc by microscope and microstructure by transmission electronic microsope1.3.2 detect the expression of VEGF in the intervertebral disc by SABC imunohistochemical staining method1.4 instruments: VEGF monocolonial antibody; SABC imunohistochemical kit and DAB coloration regent; poly - lysine; Leitz 512 mode microtome; BX60F/PM Olympus micrographier system; and Metamorph/DP10/BX51 colorful pathologic analysis system.2 Clinical research:2. 1 specimen selection and grouping; 38 intervertebral discs of different patients were selected and divided into four groups:normal group: normal intervertebral disc of patients who suffered from lumbar vertebral attack.inflate group: disruption of inner layer of annular fibrosus.protrution group: disruption of whole annular fibrosus, the nucleus pulpo-sus just beneath the ligament longitudinale posterius.liberation group: the nucleus pulposus was isolated into the vertebral canal.2. 2 method of detect : ELISA method was used to detect the presence of VEGF and PDGF in the intervertebral disc of different patients.2. 3 instruments: Elisa kit of VEGF and PDGF; extraction solution; MK -2 mode well scanner; Sartorius mode electronic scale; whizzer; microamount transmitter.ResultsIn adult and old rats notochordal cell and cartilage cell appeared degeneration and necrosis, collagen appeared proliferation irregular and gap. The elder age the more degeneration. The VEGF - positive cell ratio observed significantly varied as the rat aging. Histological structure of annulus fibrosus changed,and the quantity of chondrocyte decreased and collagen were thick, hyperplastic and...