Experimental Research Of Trypsin Inhibitor For Prevention Of Bacterial Translocation In Acute Necrotizing Pancreatitis

PrefaceAcording to the acknowledge of acute necrotizing pancreatitis, we know that pancteatic microcirculation dysfunction, released inflammation medium and cyto-kine, epithelial cell apoptosis are playing important roles in the duration of acute necrotizing pancreatitis. Those cause the ultrastrusture changes of gut mucosal including opened tight junction and increased permeability. So the patients with ANP may be more exposed to the impaired gut mucosal barrier dysfunction. This cause the bacteria and endotoxin in gut translocate to blood in vein and other area. It is one of the important reasons that the ANP patients died latter time. The objective of this experiment is to establish rats model of ANP, and trypsin inhibitor is given to the models. At proper time we culture the ascites, blood in vein and mesenteric lymphatic nodes. The number of bacteria will be taken and analysed by statistic methods, so we can conclude that whether trypsin inhibitor could decrease the bacterial translocation in ANP.Experimental MaterialsExperimental instruments: Blance ; Tub for grinding tissue; Culture Box.Experimental drugs: 3% taurocholate (Sigma Company,USA) ; trypsin inhibitor (GuangDong Tianpu).Experimental animals: Eighteen male healthy SD rats weighing (250 ~ 300g).Experimental MethodsEighteen Sprague - Dawley rats were randomly classified into three groups. Group I was only performed laparotomy by touching pancreas three times. Group II and group IE were control and experimental teams. The latter two groups were induced in Sever acute pancreatitis by injecting 3% taurocholate under the membrane of pancreas through fine needle. Rats in group II and III were administrated of 3ml normal saline at 6th and 12th hours after operation. Rats in group III were got administration of 100 thouand unit trypsin inhibitor , that volum of the trypsin inhibitor solprotaese injibitoron was 3 ml. All the rats in three groups were normaly feeded by water and food. Every rat was killed at 18th hours after operation. Aseptic laparotomy was performed and the samples of astices and blood in vein were obtained. lOul of the samples was taken and added 500ul NS. After full vibration, 1ul of the solprotaese injibitoron was taken for culture. The MLN was taken and weighted, then put into tissue grinding tube where there was 1ml NS. After complete grinding , 1ul of the mixture was taken to culture. The bacteria - colonies of all the samples were calculated after 24 hours culture. The kinds of the bacteria were decided. The pancreatic tissue was taken for pathology test, so to decide the typ of the pancreatitis.Experimental ResultsThe number of the bacteria - colonies:Group astices( 10~6/ ml) blood in-vein( 10~6 /ml) MLN( 10~6/g) I 0.000075 ±0.000042 0.0001 ±0.000063 32.67 ± 14.87 II 1.31 ±0.14 0.89 ±0.12 348.67 ±40.89 III 0.4 ±0.007 0.26 ±0.07 31.33 ±25.62The results of each samples were done T - test each other. Every two groups had the result; P <0. 01 in both astices and blood in vein teams. About...