The Dynamic Changes Of Nuclear Factor-κB In Lung Tissue Of Newborn Rats With Hyperoxia-induced CLD

The current version does not support copying Cyrillic text to the Clipboard. You should activate the programThe pups of model group were placed in 90% oxygen continuously with dioxide concentration lesser than 0. 5% , and pups of control group remained in room air. Nursing mothers were rotated between oxygen-exposed and room air rats every 24 hrs to avoid oxygen toxicity and to eliminate maternal effects between groups. Temperature and relative humidity were maintained at 22X. -25T1 and 50% ~70% respectively. For a daily 30min period, the box was opened to provide fresh food and water, weight the rats, and exchange the nursing mothers between the two groups.2. Sample collection and treatmentAnimals were killed by an intraperitoneal injection of pentobarbital sodium on day 1, 3, 7, 14, 21 respectively. The lung tissues were collected and stored in 10% formaldehyde, dehydrated by alcohol, cleared by dimethyl benzene, then paraffin imbedded. Tissue sections were made 4jxm thick and stained by H. E..3. Experimental methods and analysis markerImmunohistochemistry (SABC method) was used to detect the location and expression of NF-i