Study On Effects Of Extractions From Fruits Of Panax Ginseng And Panax Notoginseng On Promoting Angiogenesis Of Ischemic Myocardium Of Rats After AMI And Intervening Culturing HUVEC In Vitro

Objective: Study the affections on internal associations of removing blood stasis and promoting tissue regeneration and inducing generating collateral branch blood vessels , improving blood perfusion and diminishing area of ischemia of ginseng and notoginseng, which are Chinese traditional medicines of supplementing qi and activating blood circulating. Reveal the scientific meanings and processing mechanisms of Chinese traditional medicines of supplementing qi and activating blood circulating on removing blood stasis and promoting tissue regeneration.Method: In cellular experiment, the HUVEC were selected to be the models and were observed their proliferation , secreting VEGF , VEGF mRNA expression. In animal experiment, rat AMI models were established and were killed after 2 weeks.The expressions of MMP, TIMP, VEGF in ischemia myocardium and expression of VEGF in serum were observed through the ways of immunohistochemistry and ELISA. In clinical study, 128 cases of AMI have been collected through reviewing analysis and observed the major clinical typing and treating conditions of Chinese drugs.Results: In cellular experiment, Ginseng and notoginseng can promoting the HUVECs to proliferates secrete and express VEGF markedly. In animal experiment, Ginseng and notoginseng can promoting the expressions of VEGF and MMP in ischemia myocardium. In the same time, Chinese traditional medicines of supplementing qi and activating blood circulating can inhance the concentration of VEGF in serum.. In clinical study, qi deficiency syndrome accounts for 39.1 % of deficiency syndrome in origin, syndrome of deficiency of both qi and yin accounts for 30.5%. Blood stasis type accounts for 28.9% of superficiality excess syndrome, bloodysputum and blood stasis obstruction type accounts for 56. 3 % , stagnancy of qi and blood stasis accounts for 5. 5 % . In clinical treatment, 78.1 % of patients were treated with both qi-reinforcing drugs and blood-activating drugs through vein, 9.4% of patients were treated with qi-reinforcing drugs and 5.5% of patients were treated with blood-activating drugs. In oral decoction treatment, the frequency of using Chinese drugs of supplementing qi and activating blood circulation is the highest.Conclusion: Deficiency of qi and blood stasis is the major clinical typing and...