Protective Effects Of Kangshouling On Scopolamine-induced Amnesia In Rats And Its Molecular Mechanisms

Kangshouling (consisting of Radix Polygoni Multiflori Preparata, Radix Astragali, Radix Codonopsis, Radix Angelicae Sinensis, Ganoderma, Rhodiola crenulata, et al) produced good therapeutic effect on Alzheimer' s disease. In order to elucidate the mechanisms of Kangshouling on learning and memory, the effect of Kangshouling on scopolamine-induced amnesia rats and on hydrogen peroxide-induced cultured SH-SY5Y cells are obeserved.In this paper, effect of Kangshouling on scopolamine-induced amnesia in rats is evaluated by Morris water maze test. The results exhibit that 30-days administration with Kangshouling to rats attenuate memory impairment by scopolamine, indicated by shortening escape latency, swimming distance and the time for first reaching the platform(.P<0.05,P<0.01,P<0.01), increasing the swimming time of amnesia rats in the quadrant where platform was placed and frequency to passing through the position where platform was placed(P<0.05,P<0.05). The results also indicate scopolamine, a traditional muscarinic receptor antagonist, evaluated oxidative status in brain characterized by increasing the content of MDA(P<0.01) and inactivation of SOD and GSH-Px(P<0.05,P<0.05) and consequently inhibition of ATPase (P<0.01)in the hippocampus and cerebral cortex in amnesia dose. But Kangshouling reversed it by lowering MDA(P<0.01) and increasing the activities of SOD and GSH-Px(P<0.05,P<0.05) and also enhancing ATPase activity(P<0.01).To investigate protective effect of Kangshouling on the damage of neuroblastoma SH-SY5Y cells cultured in vitro introduced by H2O2, we observed that the medical serm of Kangshouling can attenuate the damage of neuroblastoma SH-SY5Y cells by increasing significantly the survival rate(P<0.01), decreasing the changes in cell morphology, and lowering LDH release from cells into culture medium(P<0.01), suggesting a cognitive improvement of Kangshouling, at least partially, via its antioxidant activity.In conclusion, Kangshouling improve the ability of learning and memory of scopolamine-induced amnesia rats and its related mechanisms at least relevant to its antioxidant effect. In addition, The medical serm of Kangshouling can attenuate the damage of neuroblastoma SH-SY5Y cells which maybe also related to its improvement in learning and memory.