A Study On The Relativity Between The Concentration Of Circulating Non-Host Fetal DNA In Pregnant Women Plasma And Pre-Eclampsia

Objective To probe the relativity between the concentration of fetal DNA in pregnant women plasma and preeclampsia.Methods: 30 cases, bearing a male fetus by B-ultrasonic, of preeclampsia and of normal pregnancy each were selected for a case-control study matched for gestational age. The bloods of the patients with preeclampsia were sampled at 20-week gestation, in the time affected by preeclampsia and, 1 hour, 3 hours and 6 hours after labour. The bloods of the normal pregnancy were sampled at the same time. SRY levels in maternal blood were quantified with a fluorescent quantitative polymerase chain reaction (QF-PCR), the concentration of fetal DNA with SRY gene therein being measured. The endotheliotoxin level is measured with radioimmunoassay(RIA). The difference of the concentration of fetal DNA in pregnant women plasma and the endotheliotoxin level between the two groups was compared using SPSS software variance analysis, Q test and T test.Results: During attack of preeclampsia, the mean of fetal DNA concentration of the 18 light preeclampsia cases in control group is757. 66 ± 357.13copy / ml and that of 12 grave cases is 1284. 93 ± 572. 89copy / ml; while that of the contrast group is 319. 22 ± 98. 85 copy / ml. Prior to the clinical and physical symptoms of preeclampsia, at about 20-week gestational age, the DNA concentration for light preeclampsia cases is 265. 85 ± 78. 63copy / ml and that for gravy cases is 396. 32±90. 51; while that for the contrast group is 165.11 ± 43.12copy / ml. At the same time, the endotheliotoxin concentration in maternal blood is measured, the mean value for light preeclampsia cases being 74.15 ± 13. 75 ng/l and that for gravy cases being89. 38 ± 31. 72ng/l; while that for the contrast group being 50. 4 ±10. 8 ng/l. The variance analysis and in-pair Q test indicate that the fetal DNA and endotheliotoxin concentration in maternal blood of light preeclampsia cases is remarkably higher than that for the contrast group, and the same with grave preeclampsia cases against light cases. Both the difference is significant (at p<0.001). Positive relation was observed between the fetal DNA concentration of preeclampsia and endotheliotoxin concentration ( r=0.748, p<0.01 ) .The mean concentration of cell-free fetal DNA in maternal blood measured at 1 hour, 3 hours and 6 hours after labour of the control group are 198.2± 50.8 copy / ml, 96.5 ± 39.4copy / ml, and 52.1 ±15.3copy / ml respectively, none of the cases having 0 concentration after labour. However,...