| Objective: To observe the protective effects of ciliary Neurotrophic Factor(CNTF) on optic nerve and retinal ganglion cells(RGCs) in rat eyes with acute ocular hypertension. Methods: 27 Wistar rats without eye diseases, 3 rats were normal group, 24 rats were divided into 4 teams by 1, 3, 7, 14 days(â… ,â…¡,â…¢,â…£), according to survival days. Eyes of every rat were divided into two groups, right eyes were control group(A) by contrast with left eye as CNTF group(B) , there were 8 groups altogether, each group has six eyes. Animal model of acute ocular hypertention was induced in each eye of 24 rats by forcing perfusion of balanced salt solution into the anterior chamber until the IOP reached 100mmHg and sustained for 60 minutes. Two days before ocular hypertention induced, rhCNTF(0.5μg/5μl) was injected into the vitreous of left eye only once as CNTF group, while sodium phosphate solution (5μl) was injected into the vitreous of right eye as control group. At the end of observation period the animals were killed , the eyeballs and intraorbital optic nerves were enucleated. The retinal ganglion cells were expressed by HE staining and SABC method labeling Glutamate, optic nerve axons were expressed by staining myelin sheath. The pathological changes of the retinals and the optic nerve axons area of optic nerve were examined with light microscopy and transmission electronic microscopy(TEM). Results: The RGCs density, the optic nerve axon area of optic nerve were decreased in ocular hypertention eyes, that of CNTF groups were more than that of control groups, there were significant difference (P<0.01). The Glutamate expression densities of RGCs of CNTF groups were less than that of control groups, there were significant difference(P<0.01). By the TEM, the RGCs morphogenesis of CNTF group was better than that of control group. Conclusion: CNTF could provide neuroprotection for RGCs and optic nerve in a rat acute glaucoma model.
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