| Objective: To observe the acute rejection after orthotopic liver transplantation in rats between closed colony SD and inbred line Wistar, to prove rejection rank of the two strains and to provid model or experimental basis for studies of the acute rejection or immunologic tolerance. Methods: (1) The models of orthotopic liver transplantation in rats were established between closed colony SD and inbred line Wistar. (2) The postoperative rats were randomly divided into five groups(n=7/group). Group A: the blood and liver samples were collected 2 days postoperatively. Group B: the samples were collected 4 days postoperatively. Group C: the samples were collected 7 days postoperatively. Group D: the samples were collected 10 days postoperatively. Group E: General condition and survival time were observed postoperatively. (3) At different time postoperatively, hepatic zymogram, including serum alanine transaminase(ALT), aspartate transaminase(AST) and lactate dehydrogenase(LDH) were tested, meanwhile liver tissues were collected for histopathological studies under light microscope. (4) Mental state, response of stimulation, activity, diet, drinking and survival time were observed in group E. Results: (1) Among 38 recipients, 35 survived more than 48h, the successful rate is 92.1%. (2) As time going on, the recipients'general condition became better gradually in group E. one rat survived more than 27d, others survived more than 30d. the values of serum ALT,AST and LDH decreased significantly. the histopathologic changes of liver tissues were relieved gradually. Conclusions (1) The key of establishing successful models include high quality donor liver, practiced microsurgical techniques and short anhepatic phase. (2) The model in our experiment appeared acute rejection in the first 4 days after operation and appeared immunologic tolerance 7 days after operation.The model can not be used to study acute rejection or immunologic tolerance effectively.
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