The Status Of FHIT Gene In Human Esophageal Carcinoma

Objective To investigate the role of FHIT gene in oncogenesic and progress of human esophageal carcinoma and to explore the relationship of FHIT gene with the development of esophageal carcinoma, we study the different expression level of the FHIT gene in human esophageal carcinoma tissues and esophageal adjacent tissues. We hope to be able to provide a new method for early diagnosis treatment and prediction of esophageal carcinoma.Methods . Tumor tissues and adjacent normal tissues in forty esophageal carcinoma cases were checked for the expression of the FHIT gene expression. Samples were divided into two groups: carcinoma and normal group, and were embeded in paraffin. By histochemistry analysis, We checked the FHIT protein. Those stained in plasma are positive cells, with the computer image analysis system, we counted the numbers of positive cells/mm2 .Results The expression level of FHIT gene was found to be significantly lower in esophageal carcinoma tissues than in normal tissues (p<0.05), and the lower expression is also closely associated with cancer cell differention, lymph node metastasis and the rate of five-year survival after surgery of esophageal carcinoma.Conclusion FHIT gene is a candidate tumor suppressor gene, which is found in 1996 by Ohta. By histochemistry analysis forty esophageal patients were examed for FHIT gene expression, and compared the different expression in tumor tissues and adjacent normal tissues, different degree of tumor cell differention and its relationship with the rate of five-year survival, found thatrwith the development of esophageal carcinoma, the expressionof the FHIT gene showed a trendence to become lower. Thus we further confirm that FHIT gene is a tumor suppressor gene and its lower gene expression is closely associated with the development of esophageal carcinoma. The check of the FHIT gene is clinically important to early diagnosis treatment and prediction of esophageal carcinoma.