In Vivo Phycocyanin Treatment Of S₁₈₀ Bearing-mice: Increase In Immunity Function And Inhibition Of Tumor Growth

Objectives: 1,To study the effect of Phycocyanin(PC),with or without Cyclophosphamide(CY), on the growthinhibition of a sarcoma cell line S180 cells and on the immunityimprovement of S180-burdened mice. 2 ,To observe theanti-tumor mechanism of PC and the optimal anti-tumor dosageof PC. To find out the side effect of PC.Methods: S180 cells, a sarcoma cell line, were injectedintraperitoneally into Kunming mice for cell passage culture.Ten days later, S180 cells, together with ascites, were collectedfrom the Kunming mice, washed and resuspended in normalsaline solution (NS) at the concentration of 2×10~7 cells/ml.Then, each 0.2ml of the S180 cells was inoculatedsubcutaneously into the right axilla of each test mice. Thesemice were randomizely devided into 4 groups: PC-treatmentgroup(including 50,75,100mg/kg/d subgroups), CY-treatmentgroup(20mg/kg/d), PC with CY-treatment group andNS-treatment group. The NS-treatment group andnon-inoculated mice group served as control groups. 24 hoursafter the inoculation, PC or CY or PC plus CY or NS, at a finalvolume of 0.2ml, were injected to each mouse intraperitoneally,respectively, and consecutively for 7 days. The mice were killedafter the seventh treatment, the peripheral blood and the wholetumor were collected. Each mouse's body weight and tumorweight was detected and blood rutein exam was done. Spleniccells were collected and cultured with ConA for 36 hours. Theserum level of IL-2, IFN-γand TNF-αin each mice wasmeasured with a commercially available ELISA kit, accordingto the manufacture's instruction, and the result was shown aspg/ml.Results: PC could inhibit the growth of S180 cells at thesethree dosages: 50mg/kg/d, 75mg/kg/d and 100mg/kg/d, and theinhibition rate was 41.41%, 37.37% and 36.36% respectively.PC-treatment increased WBC counts in PB of tumor-bearingmice. CY-treatment markedly inhibited the growth of S180 cells,but reduced WBC counts. The results also showed thatPC-treatment and tumor-bearing situation increased the spleenweight and enhanced the splenic T lymphocyte proliferationinduced by ConA. PC-treatment didn't reduce the weight oftumor-bearing mice. Among the three dosage, the dosage of50mg/kg/d was shown to have the best effect in the abovementioned parameters, as well as in the increase of IL-2, IFN-γand TNF-αsecretion. The PC dosages at 75mg/kg/d and100mg/kg/d just increased the serum level of IFN-γand TNF-α, without the increase of IL-2. Compared with PC-treatment,CY-treatment diminished serum level of IL-2, IFN-γand TNF-α. PC(75mg/kg/d) combined with CY-treatment increased the...