| High morbidity of diabetes mellitus(DM) has gradually become a globality public problem. At the same time, hepatocholepathy interrelated with DM is also very popular and it makes a big challenge to the clinic surgery. So we try to establish an animal model to reseach it. After comparing the control with rosiglitazone group, we endeavour to provide the experimental theory about treatment and prevention of diabetic hepatocholepathy.Materials and Methods:1 Set up model of diabetic hepatopathy rats1. This study was conducted on 40 Sprague Dawley rats , 10 weeks old, 20 rats were male and 20 rats were female. These rats were divided into eight groups: (l)two control groups(including 5 male rats and 5 female rats): intraperitoneal injection of 0. 1M, pH 4.5 sodium citrate buffer; (2) two common DM groups (including 5 male rats and 5 female rats): treated with a single intraperitoneal injection of STZ (60 mg/kg,diluted in 0. 1M, pH 4. 5 sodium citrate buffer) ; (3)DM subgroup(5 female rats) : treated with a single intraperitoneal injection of STZ (50 mg/kg, diluted in 0. 1M, pH 4.5 sodium citrate buffer) ;(4)severe DM groups (5 male rats) : treated with a single intraperitoneal injection of STZ (100 mg/kg, diluted in 0.1M, pH 4.5 sodium citrate buffer);(5)two rosiglitazone treatment groups (including 5 male rats and 5 female rats): treated with a single intraperitoneal injection of STZ (60 mg/kg, diluted in 0. 1M, pH 4.5 sodium citrate buffer). After 3 days, the rats was taken with rosiglitazone(lmg/kg/d) everyday.2. Urine glucose of rats were tested daily. If urine glucose was more than ++++, 1 ~ 3 iu of protamine zinc insulin was injected subcutaneously except the severe DM group. 10 weeks after treatments described above , blood samples were taken from vena cava of rats for the measurements of glucose. The rats liver samples were stored under 2. 5% glutaraldehyde .2 Preparation the sample of the transmission electronmicroscope1. fixed, flushed, stained and desiccated the constitution;2. embed;3. Sectioned;4. draged the block;5. stained the ultrathin section;6. used the Dutuch Philip TECNA10 transmission electron microscope to observe the sample.Results:1. Rat body weight: Compared the control, the body weight of the DM groups obviously descended druing the 10 weeks. And the body weight ofrosiglitazone treatment groups slightly increased when compared with the DM groups.2. The blood glucose of the rats: Compare with the control, the blood glucose of DM group was significant high, and it was more obvious in male rats. Compare with the control, the blood glucose of the DM subgroup was same and blood glucose of rosiglitazone treatment group was still high as DM group.3. Hepatocyte and cholangiole ultrastructure of Sprague-Dawley rats:(1) Hepatocyte ultrastructure of normal rats was clear and distinct. Cytoplasm was plenty and it abundantly contained many kinds of organelle. There were lots of mitochondria which had full cell membrane .The cristae in the mitochondria were long and clear. Endoplasmic reticulum arrayed in order as parallel. Nucleilus was big and round ,and its membrane was integrity. Hepatin could be seen. Cholangiole was not outstretched.(2) Hepatocyte ultrastructure of common diabetic rats was out-of-order. Mitochondria became swell, blurry or atrophic indeed, part of them is amalgamative. The cristae in the mitochondria were mixed together and could not be identifiable. Endoplasmic reticulum ruptured and arrayed mussily. Nucleilus was slight stained. Fatty degeneration was seen in cytoplasm. Cholangiole was obviously outstretched, and the wall of it is unclear. Some amorphism substance were found in the cavity of cholangiole.(3) Hepatocyte ultrastructure pathological changes of diabetic subgroup rats is worse than normal group' s and better than DM group' s. Many vacuoles were found in cytoplasm. Mitochondria were blurry. Endoplasmic reticulum slightly ruptured. Cholangiole was mildly outstretched.(4) Hepatocyte ultrastructure of severe DM group was destoried much more badly than Dm group. Mitoc...
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