Studies On MRNA Expression Of Multiple Cytokines In Bone Marrow Mesenchymal Stem Cells From Patients With Multiple Myeloma

ObjectiveTo study the abnormality of multiple cytokine mRNA expression of bone marrow mesenchymal stem cells (BM-MSCs) from patients with multiple myeloma (MM), as well as to explore the role of these abnormalitis in the occurrence and development of MM. MethodsSemi-quantitiative RT-PCR was used to detect the mRNA expressions of IL-1β, IL-6. SCF, TPO, G-CSF in BM-MSCs from MM patients. ResultThe ratios of multiple cytokines mRNA level to β-actin mRNA (Rm) of BM-MSCs in MM patients were detected and were compared with those of normal controls and other hematological malignancies.The Rm ratios of IL-1β, IL-6, SCF and TPO in MM patinets were 0.6922 ±0.1646, 0.9221 ±0.1234, 0.7041 ±0.1109, 0.6770 ±0.1777, respectively.The Rm ratios of IL-1β, IL-6, SCF and TPO in other hematological malignancies were 0.1821 ±0.0593, 0.2371 ±0.0491, 0.3222±0.0436,0.5400±0.2511, respectively.The Rm ratios of IL-1β, IL-6, SCF and TPO in control group were 0.2849 ±0.1464, 0.4574 ± 0.0644, 0.4009 ± 0.0971, 0.3342 ± 0.1182, respectively.MM patients had higher mRNA expression levels of IL-1β and IL-6 than that of normal controls and other hematological malignanciesFor the expression of SCF, There is no significant differences between the transplanted patients and the normal controls ( P> 0.05 ), but we could see a the significant difference when the primary MM and the relapsed MM constrasted to the normal controls( P<0.05 ). The expression difference is significant between the MM patients and other hematological malignancies ( P<0.05 ) .For the expression of TPO, There is a significant difference between the MM patients and the normal controls (to the primary MM P<0.01, the other P<0.05 ) .When we saw a significant difference between the primary MM and other hematological malignancies( P<0.01 ), we didn't see the same when the relapsed MM and the auto-transplanted MM contrasted to other hematological malignancies .We could not find any G-CSF mRNA expression in all study groups within the sensitivity limitation of our methods.ConclusionIL-1β, IL-6, SCF and TPO are up-regulated at mRNA level in in vitro cultured BM-MSCs from MM patients and may play an important role in the disease pathogenesis.