Experimental Study On Antitumoral Efficiency Of Foreign Gene Transfection Mediated By Ultrasound Enhanced Microbubble Destruction In Vitro And In Vivo

[Objective] We tested the transfection efficiency and the expression of foreign genes in in vitro and in vivo , to explore the possibility for transfer of theraputic genes into targeted tumor cells or tissues. [Methods] We transferred the green fluorescence protein (GFP) gene and plasmid pcDNA3/IFN- γ into human colon cancer cell line SW480 in vitro and the transplantd hepatic cancer (walker₂56) models in Wistar rats in vivo, by ultrasound irradiation mediated air filled albumin microbubbles or SonoVue microbubbles destrction. Expression of the GFP gene was quantified by laser confocal microscopy analysis. Cell viability was assayed by Trypan Blue staining. Expression of plasmid pcDNA3/IFN- γ was quantified by ELISA and immunohistochemistry methods.[Results] Ultrasound combined with microbubbles can enhance gene transfer in cultured cells, but the effect is vary according to the ultrasound condition and microbubble concentration. Optimal gene expression occurred with microbubble at the concentration of 10 % and ultrasound parameter at 0.75W/cm² for 40 second. Under this condition, the...