An Experimental Study On Placing BFGF At The Position Of Denervated Skeletal Muscle To Affect The Muscle Satellite Cell Proliferation And The Muscle Atrophy

BackgroundIt is always a difficult problem in clinical that denervated skeletal muscle affect the limbs function. Especially the small muscle has been irreversible damaged before the peripheral nerve reinnervating, which will affect delicate limb action seriously. Researchers try to resolve this problem by many methods such as electro - stimulation , passive motion , medicine, sensory neuron implantation and gene engineering etc. With the developing of molecular biology and micro -surgery technology, people have make headway. But in clinical there is no key breakthrough. Skeletal muscle atrophy is still a neck nowadays.Generally thinking the losing of nerve - nutritious factor and limb deposing lead to muscle atrophy. The greatest change in denervated skeletal muscle is the losing of nerve - nutritious factor, which lead to changing of muscle structure , physio - biochemical and metabolism ect. Studies have proved that muscle satellite cell is a potential - ability cell. It can change into muscle cell. When skeletal muscle is damaged or denervated, muscle satellite cell can be activated. While recent researchers on bFGF affecting muscle atrophy is few overseas, and no relative systematic report in China. Hense, research on exogenous nerve nutritious factor - - - bFGF affecting denervated skeletal muscle atrophy, and bFGF activate muscle satellite cell and promote its proliferation, are very important to prolong muscle atrophy. Its important sense to gain time for reinnrevated target organ.ObjectiveExogenous nerve nutritious factor - - bFGF is encapsulated to the dener-vated skeletal muscle in order to activate the muscle satellite cell and accelerate its proliferation, reduce muscle fiber atrophy and inhibit inter - fiber connective tissue proliferation so that muscle atrophy can be lightened.Methods1. Animals grouping and operation methods:20 Wistar mouse are divided into experiment group and control group randomly by means. Break the left lower limb sciatic nerve, take gastrocnemius. The experimental gastrocnemius is encapsulated with bFGF. The control group is encapsulated with physiological saline in relative consistent part. After 14 and 30 days observe the target.2. Reagent:The silia gel tubes contain bFGF and physiological saline relatively.3. Observation contents;(1) Fibre vibration range evaluation(2) Muscle wet weight evaluation(3) Histological evaluationH. E. staining and Mallory staining AgNO3 staining PCNA staining(4) Electron microscope sample(5) Statistic analysisResults1. Macroscopic evaluation;Both gastrocnemius groups have atrophy. Especially the control group 30 days after operation is much more obviously, the gastrocnemius are firmly adhered with tissues around. 2 groups comparing; 14 days later, the control group muscle is smaller, and adhere with around tissue more seriously. But the 30...