Effect Of α-lipoic Acid In Free Fatty Acid-induced Insulin Resistance

ObjectiveTo prove free fatty acid - induced insulin resistance with euglycemic - hy-perinsulinemic clamping and assess the effect of α- lipoic acid in this resistance.MethodsIndwelling catheters were inserted into the right internal jugular vein for infusions and the left carotid artery for sampling in 20 female Wistar rats. After 3 days period of postsurgery recovery, the rats were randomly divided into 4 equal groups, one of which received IH infusion (20% intralipid + 20u/ml heparin, 5.5ul/min) , one of which received a -lipoic acid, one of which received IH + α-lipoic acid infusion,another group was a saline(5ul/min) -treated control. 2h euglycemic - hyperinsulinemic clamping was made after 5h infusion. Blood samples for insulin and FFA were taken at 0min,280min,290min, 300min,340min,380min,400min,410min,420min.ResultsIH elevated plasma FFA level from 515.54 ±391.20umol/l to 1623.72 ± 698.21umol/l, and there was significant difference between the IH group and the control group ( P < 0.05). But there was no significant difference between IH...