| The malignant tumor is the disease of endangering the human health badly. Primary liver cancer is one of them. Although the diaginose and the treatment have been improved time and again, there are also a great heath rate. The suicide gene therapy of the tumor has represented the good application prospect as a kind of new approach of treating tumor. But suicide gene therapy alone lie in great defect, because some residual cells will lead to tumor recurring after treating it. Because of the important function of bystander effect and its immunological mechanism, it is a key to strengthening bystander effect by improving the immune system to kill residual tumor cells and cure tumor. And the function of improving immunologic function and anticancer of Liuwei Dihuang Dolus has been comformed in many research. It will be studied to combine Liuwei Dihuang Dolus and suicide gene therapy in treating the tumor of KM mice in this study.Objective: To construct a new cell stain by transfect tk gene to H22 cell stain and name it H22/tk. Then use it for treating tumor in vivo and observe the curativeeffect of treatment by combining Liuwei Dihuang Bolus and GCV. And study the mechanism by immunohistochemical and TUNEL method. Provide requirement for gene therapy of tumor by combining traditional Chinese and western medicine.Methods: Based on PT67/tk cell stain has been construction by DNA recombinant technique, then determining vival titer by infecting NIH 3T3 cells. H22 was infected with the recombinant retrovirus. The positive clones were obtained after G418 selection and named H22/tk. The morphological changes after GCV treatment were observed with microscope. The liver tumor model on the KM mice were made in vivo by inoculate the cell mix whick comprise 80% H22 and 20% H22/tk. Give a treatment by injecting pro-drug GCV, and combined with Liuwei Dihuang Bolus. Observe the weight of tumor after 16 days and histopathological expression of the tumors were observed in every group. Observe...
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