Dynamic Studies On The Effective Components Of Genuine Citrus Grandis

Objective:To study the relationship of dynamic effective components with medicinal part, different native areas, fertilitizer of Tomentulosus Exocarpium Citri Grandis (TECG) with modern analysis equipments which including HPLC, GC-MS, UV etc. The results indicate the dynamic tendency of the chief effective components. These data provide a scientific information for the plan on the techniques of cultivation, period of collection, comprehensive application and standardization of cultivation according to the Standard Operation Pratice(SOP) and Good Agriculture Practice(GAP) of TECG . Methods:The content of Pb was determined by GB/T5009.12-1996 method. The content of Cd was determined by GB/T5009.15-1996 method. The content of Hg was determined by GB/T5009.17-1996 method. The content of Cu was determined by GB/T5009.13-1996 method. The content of As was determined by GB/T5009.11-1996 method. The content of total flavonoids was determined by spectral photometric analysis. The content of naringin was determined by RP-HPLC. The content of rhoifolin was determined by RP-HPLC. The fingerprints of TECG was studied by RP-HPLC. Results: 1 The study of dynamic different medicinal parts of Exocarpium Citri Grandis (ECG)The comparative study of different medicinal part of ECG indicates that the content of narigin is fruit>flower>fruit carpopodium>leaf>stem. The comparative study of volatile oil components of different medicinal part indicates that there are obvious differences in the content and types of composition in each medicinal part of ECG Limonene is the main composition of fruit volatile oil, Nerolidol is the major content in the flower volatile oil. Phytol and Hexadecanoic acid are the largest amount content in the leaf and stem volatileoil.2 The dynamic study of different native placesThe heavy metal of TECG in 16 cultivated areas is obviously less than the limited quantity ruled by medicinal plants and preparations import and export green trade criteria. The heavy metal of leaf is also less than the criteria except of Cu. The total flavonoids content of fruit in Iijiayuan and Tianzhizhang president nearby is higher than the other 14 areas, and the total flavonoids of leaf in Tianzhizhang president nearby is also very high.3 The influence of fertilizer on the dynamic active componentsAs the fertilizer is made up of organic fertilizer (2.5kg) + urea(109g) + Ca₃(PO3)₂(143g) +K2SO4(150g), the total flavonoids and naringin content is the highest, and the fingerprint also indicates the naringin and rhoifolin in fruit and leaf are also the highest, however, comparing the fertilizer one with the without fertilizer one ,the latter content is higher than the fomer one.4.The dynamic growing periods of leafThere are two highest value of total flavonoid and naringin of leaf in one year growing periods.The content of active component has the largest value in April and December, the former total flavonoid value is higher than the latter, the content value fallen to the lowest value at the beginning of July. 5 The dynamic study of active component with fruit-ageAs the fruit grows up successively, the content of total flavonoid and narigin will reduce, this indicates that the content has a negative line with fruit-age. The chemical composition of volatile oil has some differences within 90 days fruit-age. Limonene, β—Myrcene and γ-Terpiene are the main component in volatile oil. The content of Limonene has two highest value at 55 days and 83 days fruit-age. Conclution:1 There are distinct differences in the content of active components of different medicinal part of ECG, there are also obvious difference in the content and types of composition in each medicinal part of ECG, this indicates that the active components are dynamic distribution in morphological of ECG2 The soil suitable for the growth of ECG is acid. The heavy metal of TECG in 16 cultivated areas is obviously less than the limited quantity ruled by medicinal plant and preparations imports and exports green trade criteria. There are distinct differences in the content of active components of 16 cultivated areas, the fruit in the area which administer attentively has good quality, this indicates that the base of TECG should be strengthen administration, and this will get the good quality and large quantity of TECG3 As the fertilizer is made up of organic fertilizer(2.5kg)+ urea(109g) + Ca3(PO3)2 (143g)+K2SO4(150g), the total flavonoids and naringin content is the highest, this will provide scientific information for fertilizer plan.