The Comparative Research On The Methods Of Obtaining Decellularized Tissue Engineering Valves

Objective Tissue engineering of heart valves should avoid the disadvantages of conventional prostheses. The purpose of this study is to seek better method of making tissue engineering of heart valves by testing different decellularization procedures for their potential of cell removal and their ability to preserve the matrix.Methods Specimens of porcine aortic valves were randomly divided into the control groups and the decellularized groups. The porcine aortic valves of decellularized groups were treated with either NaCL and sodium-dodecyl-sulfate(SDS) or trypsin or Triton-X100 with specific concentration. Tissue samples were then processed for Hematoxylin-Eosin and scanning electron microscopy and immunohistochemistric staining for major histocompatibility complex class â…  (MHC- â…  ) antigen.Results NaCL and SDS achieved only incomplete decellularization, the main components of extracellular matrix were reserved completely but the fibrous components become swelling.In contrast, trypsin removed cells completely but caused strong structural alterations.Treatment with Triton-X100 achieved both complete decelluarization and preservation of the matrix structure. The decellularized valves were reserved varied level of immunogenicity. MHC- â…  was decreased remarkably in trypsin and...