| Objective: The aim of this study is to investigate the effect of dendritic cell vaccine on glioma of rats and the difference between the results caused by different injection pathways. Methods: By stereotactic technology, the animal models were created by injecting a certain number of C6 cells into the head of right caudate nucleus of the rats' brains . The glioma antigen was made by freeze thawing of C6 cells. After proliferation by rat granulocyte-macrophage colony-stimulating factor and rat interleukin-4, identification and stimulation by glioma antigen, bone marrow derived dendritic cells was injected into rats with glioma at different times by three different pathways, intra-internal carotid artery, intra-caudal vein and hypodermic. A group of rats were put to death on the 17th day after the injection of C6 cells and the size of gliomas were measured. The other group of rats were still fed until they died naturally and their survival times were recorded. Results: From the 6th to 8th day, the projection on the surface to the myelomonocytes began to be observed. On the 14th day, most cells became suspended in the culture solution and dendritic projections were observed. The shape of the cells under the electromicroscope was very typical as dendritic cells. After being cultured 10 days, OX62 and 0X6 on the cells were analyzed by double stain immunocytochemisty. The proportion of necrosis in the gliomas which had received antigen-pulsed dendritic cells was much more than that in the control group (P<0.05), and the survival time of the treated group was longer than the control group (P>0.05). Different injection pathways did not lead to different results in the treated groups 9P>0.05). Conclusions: The vaccine based on the glioma antigen-pulsed dendritic cells can inhibit the growth of cerebral glioma in rats. Different injection pathways, intra-internal carotid artery, intra-caudal vein and hypodermic, get the same result. Hypodermic injection is safe, effective and easier to perform.
|
PDF Full Text Request