Mobilization Of Stem Cells In Patients With Acute Myocardial Infarction

Background: neovascularization is a new measure of treatment for ischemic heart disease and occlusive atherosclerosis. Endothelial progenitor cells (EPCs) circulate in adult peripheral blood (PB) and contribute to neovascularization. However, little is known regarding whether EPCs and their putativeprecursor, CD34-positive mononuclear cells (MNC^CD34+), are mobilized into PB in acute ischemic events in humans.Objective:To investigate the Mobilization of CD34-positive mononuclear cells which in the peripheral blood of the patients with acute Myocardial infarction, and study mechanism of the mobilization. Method:We selected 20 patients with acute myocardial infarction and 20 healthy as the controls, and examine the number of the CD34-positive mononuclear cells through Flow cytomery . In the mean time use ELISA to examine the plasma level of vascular endothelial growth factor, (VEGF), granulocyte macrophagecolony-stimulating factor , (GM-CSF), interleukin-3, (IL-3) , interleukin-8 (IL-8) . Data were subjected to 1-way ANOVA followed by Fisher's test for comparison between any 2 means. Use simple regression to analyze the relationship between the number of circulating MNC^CD34+ and plasma levels of Cytokines.Result: MNC^CD34+ increased after the onset of AMI and peaked on day 7. MNC^CD34+ then gradually decreased, but the number on day 28 was still greater than that on day 1. In controls, circulating MNC^CD34+ counts on days 1 and 7 were similar (113 + 8 and 117 + 13/106 WBCs). The MNC^CD34+ counts did not differ between the AMI and control groups on day 1, but they were significantly greater in the AMI group...