The Experimented Study For Mechanisms Of Antiangiogenesis Effect Of Ginsenoside Rg3 On Endometriosis

Objective Ginseng plays an important role in Chinese medicine, whichhas been used to treat tumour. It's igredient, ginsenoside Rg3, was testified toinhibit vescular endothelial cell growth and to have antiangiogenesis. To explorethe methanism and evaluate ginsenoside Rg3's effect on endometriosisantiangiogenesis, the author observed ginsenoside Rg3's influence onendometriosis rats model ectopic endometrium grafts volume and morphology,and it's influence on expression of vescular growth factors. Methods Wistar rats endometriosis models were established referring toJones methods,successful endometriosis rats were divided into control group,ginsenoside Rg3 5mg/kg group, gestrinone group, ginsenoside Rg3 10mg/kggroup each with 2 weeks, 4 weeks, 6 weeks and 8 weeks all 16 groups. Ectopicendometrium grafts volume and histomorphology were observed, vescularendothelial growth factor(VEGF) was observed in ectopic endometrium byimmunohistochemistry(SABC technique). MVD was determined byimmunostaining for CD31. Results (1) No significant difference was found in the volumes of graftsof each groups(P>0.05) and the appearance of the grafts are almost the samebetween the second and third operation of control rats. (2) No significantdifference was found in the volumes of grafts between control group and eachtherapy group on the second operation(P>0.05). (3) The volume of grafts ofdifferent therapy group on the third operation was differently reduced comparedwith the second operation, and was significantly reduced compared with thecontrol group on the third operation (P<0.05). (4) Treated for 8 weeks, thevolume of grafts of each therapy group was significantly reduced comparedwith the second operation(P<0.05). Macroscopically, flat and atrophic graftswere observed. (5) The expression of VEGF and MVD of ectopic tissues ofeach control group were no significant difference (P>0.05). (6) The expressionof VEGF and MVD of each therapy group were significantly lower than controlgroup after different period of therapy(P<0.05). (7) The volume of grafts andthe expression of VEGF and MVD of the third operation of ginsenoside Rg310mg/kg group were significantly lower than control group, Rg3 5mg/kg groupand gestrinone group(P<0.05). (8) There was significantly linear positivecorrelation between the expression of VEGF and MVD of each therapy group(P<0.05). (9) There were interactive effect between therapy length and therapydose. The volume of grafts was the smallest and the expression of VEGF andMVD were the weakest after 8 weeks therapy of ginsenoside Rg3 10mg/kg,indicating this therapy has the strongest inhibitive effect on the cyst of theectopic endometrium. Conclusions Ginsenoside Rg3 has antiangiogenesis effect inendometriosis rats'models, which was interrelated to dose and length of therapy.The study provided novel meassures and targets for the clinical therapy ofendometriosis.