Experimental Study On Nasopharyngeal Carcinoma Therapy By Transfection Of Bcl-2 Antisense Oligodeoxynucleotides

PART ONE Objective: To observe the effect of bcl-2 gene antisenseoligodeoxynucleotides (ASODN) transfection to the proliferation of thehuman nasopharyngeal carcinoma (NPC) HNE-1 cell lines. Methods: There were 4 groups in our study:control groupⅠ, bcl-2sense mixed Lipofectin group Ⅱ, half-dose bcl-2 antisense mixedLipofectin groupⅢ, whole-dose bcl-2 antisense mixed Lipofectin groupⅣ.The cell proliferation and changes of the cell morpholog were observed byelectronic microscope, MTT assay test and Cell cycle were detected byflow cytometry. Results: The trend of elongating S phase was the most obvious byflow cytometry .The evident difference had been found about the inhibitoryrate of cell proliferation between the antisense experimental group andother groups. Conclusion: bcl-2 gene ASODN transfection can inhibite theproliferation of the human nasopharyngeal carcinoma (NPC) HNE-1 celllines.PART TWO Objective: To explore the proliferating inhibition molecularmechanisms of bcl-2 ASODN transfection on the human NPC HNE-1 celllines. Methods: There were 4 groups in the test: control groupⅠ, bcl-2sense mixed Lipofectin groupⅡ, half-dose bcl-2 antisense mixedLipofectin group Ⅲ, whole-dose bcl-2 antisense mixed Lipofectin groupⅣ.The protein expressing level of PCNA and the NFкB were detected byflow cytometry and immunohischemistry assay. Results: The fluorescent intensity of PCNA protein was lowerremarkably in the antisense groups. The evident difference had beenfound about the protein expressing level of the NFкB in the antisensegroups. Conclusions: bcl-2 ASODN transfection can decrease the proteinexpressing level of PCNA and NFkB, which may be one of the mechanismsof bcl-2 ASODN transfection inhibiting cell proliferation and promotingcell apoptosis.