| AIM: The previous study showed that the expression of MUC2 protein was related with the biological behavior of gastric carcinoma. The aim of the present study is to investigate the inhibitory effect in vitro of mucin gene MUC2 antisense oligodeoxynucleotide (ASODN) on its gene expression, cell proliferation and adhesion ability on gastric cancer cells SGC7901. METHODS: Phosphorothioate MUC2 ASODN were synthesized and transfected to SGC7901 cells mediated by lipofectin. MUC2 expressed on SGC7901 cells was detected by RT-PCR. Its inhibitory effects on cell proliferation were determined by flow cytometry (FCM). Adhesion of SGC7901 cells to Matrigel after treated with MUC2 ASODN was measured by MTT method , and adhesion to endothelial cells was measured by Rose Bengal . RESULTS: 1 , As compared with the control cells, MUC2 ASODN could significantly inhibit MUC2 mRNA expression of SGC7901 cells. 2,Measured by FCM found the number of the cells treated with MUC2 ASODN were decreased compared with the control cells, left-Sidedness of G1 phase occurred sub-G1 phase. Transfection with MUC2 ASODN could inhibit the transition period of S phase to G2/M phase , and G2/M did not changed much . The apoptosis rate was about 4.38% . 3,The results demonstrated measured by MTT method at 30 min,60 min,90min, 120min that transfected with MUC2 ASODN significantly decreased adherion ability to Matrigel of SGC7901 cells compared with the control cells. 4,Data of Rose bengal method suggested the number of the cells adhere to endothelial cells ECV304 decreased compared with the control cells. 5,The test by immunohistochemical method indicated that transfected with MUC2 ASODN downregulated the expression of cathepsin D protein and nm23 protein, but upregulated the expression of E-cadherin protein . CONCLUSION: In vitro, MUC2 ASODN treatment could significantly inhibit MUC2 mRNA expression,cells proliferation, adhesion ability to Matrigel and endothelial cells on gastric cancer cells SGC7901 .
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