The First Investigation Of Inhibitory Effects On Human Gastric Cancer Cell Line By MUC2 Antisense Oligodeoxynucleotide

Objective: The aim of the present study is to investigate the inhibitory effect in vitro of mucin gene MUC2 antisense oligodeoxynucleotide (ASODN) on gastric cancer cell line. Methods: Phosphorothioate MUC2 ASODN were synthesized and transfected to SGC7901 cells mediated by lipofectin. Their inhibitory effects on cell were determined by MTT method, light and electron microscopy and immunohistochemical method. Results:1,The determination by MTT method demonstrated that MUC2 ASODN of varied concentrations could significantly inhibit the growth of SGC7901 cell lines while the control showed no such effect. The result also suggested that the inhibitory effect was dose-dependent and time-dependent. The inhibition peaked at 48th hour after transfection, and the inhibition rate reached 55% when the concentration was 0.5μM/L. 2,After transfecting with MUC2 ASODN, SGC7901 cells showed decrease in number, volume and karyokinesis, increase in necroses under light microscopy. Mitochondrion swelling, increased liposomes, myelin figures, chromatin margination were found under electron microscopy. 3,And the test by immunohistochemical method indicated that ASODN could downregulate the expression levels of MUC2 protein, MMP2 protein and CD44V6 protein , but upregulate the expression levels of p16 protein. Conclusion: The data suggest that MUC2 ASODN transfection could specific inhibit SGC7901 cells proliferation and potential of SGC7901 cells invasion and metastasis.