Study On Apoptotic Of Esophageal Carcinoma EC9706 Cells Induced By PoYuSanJieFa

Objective: As the representation of esophageal carcinoma no.l decoction to observe the effect of the apoptotic of esophageal carcinoma EC9706 cells induced by PoYuSanJieFa.Method: Examined by light microsoope> transmitting electron microsoope and flow cytornetry.Result: Apoptotic of esophageal carcinoma EC9706 cells can be induced by esophageal carcinoma no.l decoction . Under electron microscope.apoptotic modes were seen:the chromatin of the nucleus agglutinated topleces and the organelles in the cytoplasm preserved complete. The apoptotic peak was Identified by flow cytometry.And there is a significant difference between the treatment group and control group(P<0.05).And a significant difference were found between the high concentrations (1g/ml and 0.5 g/ml) of treatment group and control group(P<0.05).The results also demonstrated that inhibitory effects of recipe were associated with the high percentage of bypodiploid cells(0.1 ~lg / ml).Conclusion: Apoptotic of esophageal carcinoma EC9706 cells can be induced by PoYuSanJieFa. The results also demonstrated that inhibitory effects of recipe were associated with the high percentage of bypodiploid cells.