Study On Synthesis Of 3,6-Disubstitutedphenyl-s-Tetrazine Derivatives And Their Structure-Activity Relationships Of Anti-tumour

In order to study on the structure-activity relationship of 3,6-disubstitutedphenyl-s-tetrazine, we have synthesized a series of 1,4-hydro-S-tetrazine derivatives. Their structures were confirmed by 1HNMR, IR, MS and elemental analysis and X-ray diffraction. The antitumor activities of those compounds in vitro were tested.The target compounds were obtained via different N-substituent- α -Chloro-phenylhydrazone derivatives reaction of triethylamine in THF solvent. N-substituent- α -Chloro-phenylhydrazone derivatives were prepared from substituted benzoyl chloride or substituted benzaldehyde with substituted hydrazine. (1) two compounds of 3,6-disubstituted phenyl-S-tetrazine-l,4-dihydro-l,4-dicarboxylic acid dimethyl ester were synthesized by N-substituent phenyl carboxylic acid methyl ester- α -Chloro-phenylhydrazone derivatives, and both were new compounds. (2) Six compounds of 3,6-disubsitituedphenyl-S-tetrazine-1, 4-dihydro-1, 4-disubstituted phenyl were prepared by N-substituted phenyl- α -Chloro-phenylhydrazone derivatives, and two were novel compounds. (3) Seven compounds of 3,6-disubstituted phenyl-1,4- dihydro-S-tetrazine-1,4-substituted benzenesulfonyl were obtained by N-substituent...