Interrelationship Among The Polymorphism Of (AC)n In The 5' -end Of The AR Gene,the Erythrocyte Aldose Reductase Activity And Susceptibility Of Microangiopathy In The Patients With Type 2 Diabetes Mellitus

Objective To study the interrelationship among the polymorphism of (AC)n in the 5'-end of the AR gene, the erythrocyte aldose reductase activity and susceptibility of microangiopathy in the patients with type 2 diabetes mellitus(DM). Methods One hundred and forty cases of type 2 DM and forty-nine controls were included in this study. All these cases were the Han nationality of Guangxi zhuang autonomous region. The subjects were devided into DM without diabetic microangiopathy(NDC)(56 cases), DMAP group(84 cases), and normal controls(CON)(49 cases); furthermore, according to the type of allele, the subjects were devided into Z+2, Z-2, both Z+2 and Z-2, neither Z+2 nor Z-2(X/X). PCR was performed and PCR products were electrophoresed with 8% formamid/urea gel. AR activity(ARA) was measured by modified Sriratava method. The differences of ARA were compared among these groups. Results It showed 7 types of alleles(Z-6~Z+6). The frequency of allele Z+2 was much lower in DM group than in NDC group and CON group. ARA in DMAP group was significantly higher than that in NDC group, ARA in NDC group was significantly higher than that in CON group. Among Z-2, Z+2, Z+2/Z-2 and X/X groups. ARA was the highest in DM Z-2/ Z-2 group and the lowest in DM Z+2/ Z+2 group. Conclusion Z-2 allele might be the susceptible gene and Z+2 allele might be the protected gene. The activation of AR plays an important role in the development of DMAP. Z-2 allele may be an activator and Z+2 allele may be an inhititor of AR.