Correlation Research Of Aldose Reductase Gene Polymorphism And Type 2 Diabetic Nephropathy

Objective:The activation of Polyol pathway has been considered as one of the causes involved in the diabetic microangiopathy. In this process, aldose reductase(AR) is the first and rate-limiting enzyme, has always been the focus of different studies. Our study is to explore the relationship between AR gene polymorphism and diabetic nephropathy. Methods:A case-control study was performed to 293 subjects (84 healthy blood donors, 209 type 2 diabetics). PCR-RFLP was applied to screen the polymorphisms of the intron 8 nucleotide 95th A/C and promoter region nucleotide -12 C/G . Compare haplotype and genotype frequency in different groups, in order to determine whether the gene polymorphism is one of the reasons involved in the diabetic nephropathy. Results:(1) In groups of control, diabetic non-nephropathy, microalbuminuria, overt nephropathy, there are no differences of A/C polymorphism;In microalbuminuria group, allele G is higher than in other groups.(2) In microalbuminuria group, the frequency of genotype AA is higher than in other groups, and the same conditions occurred in the GG genotype;and there are no differences between genotypes AC and CC, genotypes CC and GG have also no differences.(3) In diabetic nephropathy group, the frequency of allele G is higher than the groups of control and diabetic non-nephropathy;the frequency of genotypes AA and GG is higher, too.Conclusion:(1)In the AR gene promoter region nucleotide -12, allele G is a risk factor of diabetic nephropathy.(2) Intron 8 nucleotide 95th genotype AA, promoter region nucleotide -12 GG are the independent risk factors for onset of the diabetic nephropathy. Whereas, there are no evidence to prove their roles in the progression of the diabetic nephropathy.