| Trichomonas vaginalis has been an important cause in the infection of the genitourinary tract, with the increasing positive rate of it in STD clinic. And trichomonas vaginalis is also considered a major risk factor in the transmission of the human immunodeficiency virus(HIV). So it is necessary for us to strengthen the study of diagnostic laboratory detection and provide a rapid and highly sensitive and specific method to screen and diagnosis trichomoniasis. Traditionally, diagnosis of trichomoniasis in women has relied on microscopic examination of wet mount preparation made directly from vaginal secretion. In comparison with culture, which is considered as "Gold standard", this technique is relatively insensitive, and the positive rate is affected by many factors such as the storage and transportation of samples, the skill and experience of microscopists and so on. While the method of culture is time-consuming, which requires frequent microscopic observation up to 7 days. Moreover culturing technique can not identify the protozoon when it is in low number or is not viable. The application of Polymerase Chain Reaction(PCR) has greatly improved the sensitivities of laboratory detecting of infectious pathogens and has opened new possibilities for the detection of trichomonas vaginalis. The aim of the present study was to study the clinical performance of Single Tube Nested Polymerase Chain Reaction (SN-PCR) in the detection of trichomonas vaginalis. To investigate the character of infection and distribution of trichomonas vaginalis in female STD patients and healthy women and to explore the infectious relationship between trichomonas vaginalis and other pathogens. Wet mount preparation, culture and SN-PCR were applied to detect the infectious situation of trichomonas vaginalis in 703 female patients with STD and 156 healthy women. Specimens were collected from vaginal vault with the help of cotton swabs. Result of test indicate: The positive rate of wet mount preparation, culture and SN-PCR was 9.20%, 17.00% and 17.69% respectively. Compared with the method of culture, the sensitivity and specificity of SN-PCR were 100% and 99.16% respectively. The differences between wet mount preparation and culture, wet mount preparation and SN-PCR were statistically significant(P<0.05). And the differences between culture and SN-PCR were not statistically significant(P>0.05).The positive rate of trichomonas vaginalis infection in female STD patients was 20.91%, which was significantly higher than those in healthy women 3.21%, and the differences of the positive numbers of trichomonas vaginalis infection between the two groups were statistically significant(Kappa=27.50, P<0.05). The differences of the positive numbers of trichomonas vaginalis infection between each STD patients and healthy women were statistically significant (each group P<0.05). The 6 STD pathogens were the dangerous factors of the infection of trichomonas vaginalis(each OR>1, P<0.05). When the comparison was done among the six kinds STD patients, the differences between Uu infection patients and NG patients were not statistically significant(P>0.05), while the differences between Uu infection patients and the other five kinds STD patients were statistically significant(P<0.05). And the differences among Gonorrhea, Ct infection, syphilis, GH and CA patients were not statistically significant(P>0.05). The differences genomic DNA consistence between standard DNA extraction method and rapid boiling method were not statistically significant(P>0.05). Our conclusions: Rapid boiling method for extraction of trichomonas vaginalis DNA and SN-PCR for detection it have the characters of easiness,... |
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