The Relationship Between Homocysteine And Stroke And The Study Of Measure The Plasma Hcy By Using Of ELISA

The risk factors for stroke are hypertension, diabetes, high level of lipid in blood, smoking and drinking. Controling these factors can prevent stroke, but some patients have not these factors. Scientists should search new risk factors for stroke, improve the cognition of the mechanism of stroke and establish new policies of prevention and treatment for stroke. Recently, with the development of method for examining measure and progress of molecular biology, many studies indicate that there is one relationship between the increase of the level of plasma homocysteine and the cause of atherosis or thrombosis. These studies suggest that homocysteinemia is the new independent risk factor for stroke. This case-control study was based 73 patients (45 males, 28 females) of stroke, average age was 59.2±11.4 years. Fifty-one cases were ischemic stroke, twenty-two cases were cerebral haemorrhage. The disgnosis accorded with the standard established on the Fourth Congress of Cerebralvascular Disease of China in 1995 and were validated by CT or MRI. Ten control subjects (5 males, 5 females) were recruited, average age was 59.1±7.6 years. In the control subjects had no diseases of heart, brain, kidney, liver, metabolization and dystrophia. Age, gender, anamnesis, blood pressure, blood sugar, blood lipide were also recorded. ELISA (STE) was used to examine the level of plasma hcy. Result: The level of plasma hcy of stroke patients (15.09±9.17 umol/L) was higher significantly than the controls(8.87±2.88 umol/L,P<0.05),the rate of hyperhomocysteinemia was 39.7%. There was no difference in the level of plasma hcy of male(14.79±9.26 umol/L)and female (15.58±9.16 umol/L), the olders (15.01±9.23 umol/L)and the youth (15.17±9.23 umol/L ) , cerebral infarction ( 14.90±8.93 umol/L ) and cerebral hemorrhage (15.54±9.89 umol/L). The level of plasma hcy had no association with the subtypes of cerebral infarction and the degrees of stroke. The level of plasma hcy of patients who had recurrent stroke (18.12±9.30 umol/L) was higher significantly than patients who had one time stroke(13.10±8.61 umol/L,P<0.05). Blood pressure, blood sugar , TC, LDL, HDL, TG, CRE, BUN were the independent variables and hcy was the variable. It suggested that the patients who had hypertension and higher TG level were easily to get homocysteinemia by used the regress analysis to analyze the relationship between independent variables and variable. Conclusions: The increase of the level of plasma hcy is a independent risk factor for stroke and recurrent stroke. The ELISA (STE) is an exact and effective measure in examining the plasma hcy which can be used in basal...