Experimental Research Of Diabetic Neuropathy Treated By Diemailing

Objects: Diabetic neuropathy is one of common chroniccomplications of diabetesmellitus. The exact pathogenesis is stillunknown and there is no effective treatment available at present. Toexamine the roles and the relationship of metabolic factors andvascular factorsin the pathogenesis of diabetic neuropathy and toprovidemore experimental evidences to expanding of diemailing'sclinical use, we investigated the effects of diemai linginjection onnerve biochemical, structural, functional deficits and decreasing bloodand plasma viscosity in Streptozocin induced diabetic rats.methods: Wistar rats were used. Diabetes was induced by singleintraperitoneal injection of Streptozocin (55mg/kg body weight)randomly chosen from the total group. Theresidual thirty received anequal volume of citric acid buffer43as nondiabetic control (NC). 48hours later, rats with plasma glucose concentration>16.7 mmol/L wereselected and divided at random into five groups: Diabetic control rats(DM1,DM2) and diabetic rats treated with diemailing (early groupEG,Late group LG). EG feeded in 8 weeks and LG feeded in 12weekswere given diemailinginjection (15mg/kg body weight) dissolved indistillationwater by gavage once a day. Residual groups received thedistillation water only. Body weight and blood glucose were measuredmonthly. After 2 weeks, motor nerve conductionvelocity (MNCV) andsensory nerve conduction velocity (SNCV) were determined insciatic-tibial nerve conducting system. After that, blood from eachgroup was obtained to measure NGF levels, blood and plasmaviscosity were executed and The residual rat's sciatic nerves wereextirpated. Semithin and ultrathin cross sections were obtained andused for morphological observation.Results: the body weight of DM1,DM2 and EG,LG rats weresignificantly reduced (P<0.05, vs NG). Plasma glucose concentrationsin diabetic rats were significantly higher than that of NG rats (P<0.05).There were no significant effects of diemailing on the bodyweight andplasma glucose level in diabetic rats. Sciatic SNCV and MNCV weresignificantly slowed in DM1 and DM2 rats (P<0.05, vs NG).Treatment with diemailing partially prevented the slowing of SNCVin diabetic rats (P<0.05), but MNCV is no slow (P>0.05). NGF levelswere increased signicantly in diabetic rats (P<0.05), NGF levels indiabetes and treatment groups were no difference. Light micrographsin DM1 and DM2 rats showed serious loss of demyelination. Electronmicrographs in DM1 and DM2 rats showed myelinated nervefibervacuole formation and abnormally. Treatment rats showedmoderate changes. NG rats, on the other hand, had mild or microlesions perhaps due to artifacts.Conclusions: Treatment with diemailing improved nervebiochemical, structural, functional deficits and blood and plasmaviscosity in experimental diabetes. These observations suggested thatdiemailing is beneficial in the prevention of diabetic neuropathy and...