| Orthodontic tooth movement base on such physiological principle,constant force is applied to a tooth result in periodontium include inperiodontalmembrane, alveolar bone and vascular rebuild in it. How to promoteperiodontium remodeling and shorten orthodontic course is a hotspot inorthodontic research. Low power laser Irradiation can increase activity ofvarious enzyme and affect permeability of cytomembrane, make the bloodcirculation establishment in regenerative organization have notable increase,can stimulate the composition of collagen, offer material foundation fororganization to restore, Can accelerate calcium quality to deposit, quicklybecome the campaign of bone cell, accelerate bone to regeneration etc.Already each clinical subject extensive application. Already at clinicalextensive use. The acceleration is being unbalanced the key which the toothmoves is the acceleration is being unbalanced the tooth to move in the processthe bone to rebuild the process. Is being unbalanced the tooth to move in theprocess the tooth week organization's blood vessel function with to beunbalanced the tooth to move in the process the bone to rebuild the processclose correlation. This experiment uses the weak laser partial illuminationrabbit experimental nature to be unbalanced the tooth to move, dyes themethod through the immunity histochemistry, is being unbalanced the toothafter the image analysis experimental nature to move in the tooth weekorganization the VEGFR-2 expression and the distribution change, the researchrabbit experimental tooth moves when in the tooth week organization theVEGFR-2 expression, the weak laser illumination moves the VEGFR-2expression influence to the experimental tooth, studies the weak laserillumination to align is unbalanced the tooth to move the influence functionwhich the tooth week organization rebuilds, provides the rationale for theclinical practice.Methods: Thirty-five rabbits were used in this study. The animals weredivided into 7 groups on average: untreated, 1d, 3d, 5d, 7d, 14d, 21d. Anorthodontic appliance, consisting of a coil spring ligated to the first molarconnecting it to an orthodontic wire ligated onto the incisors, and exerting aforce of approximately 80 g, The right was designed as experimental group,left as control group, in the self-control. Experimental group receivedirradiation of lower power laser. The animals from each group were killed atthe time discontinued. The histological sections were stained with mouseanti-rabbit VEGF monoclonal antibody to express VEGF immunoreactivity,using a SP KIT, and counterstained with hematoxylin. Omitting the primaryantibody, and substituting it with PBS tested the specificity of the immunereaction.Experimental result: In the normal tooth week organization, VEGFR-2has the expression, the limitation distributes in the newborn blood vesselendothelial cell. After the tooth week organization receives is beingunbalanced the pressure, the pressure zone 1d～7dVEGFR-2 expression andthe normal tooth week organization compare, the difference is extremelyremarkable (P<0.01);In tension area when 3d～14d, the VEGFR-2 positivereaction gradation integral and the normal tooth week organization compare,the difference is extremely remarkable (P<0.01).The crest value pressure zoneappears after the tooth stress 5d, the tension area appears in 7d.Theexperimental side and the comparison side compare pressure zone 1d theVEGFR-2 expression to be lower than the comparison side obviously (P<0.05),when 3d～5d is higher than the comparison side obviously (P<0.05).Tensionarea 3d～7d is higher than the comparison side obviously (P<0.05).Conclusion: It is unbalanced the strength to exert first does a lot oftalking in the rabbit upper jaw, the pericementum near center gap shrinksnarrowly, the blood vessel serious bearing, the blood stream reduces, rapidlycauses chemistry environment change, the VEGF expression surrenders to thestate with the oxygen deficit induces the primary factor which the VEGFR-2expression surrenders to the state, therefore after is being unbalanced the thrustaugmentation 1st d, the pressure zone tooth week organization and the normaltooth week organization compare the expression to advance obviously(P<0.01).The pressure zone 1d～7d VEGFR-2 expression and the normal toothweek organization compare, the difference is extremely remarkable(P<0.01).The tension area tooth week organization is pulled pulls, lacks theanoxemia not obvious and the VEGF expression advances not obviously, theVEGFR-2 expression changes not obviously. In tension area when 3d～14d,the VEGFR-2 positive reaction gradation integral and the normal tooth weekorganization compare, the difference is extremely remarkable (P<0.01).Theweak laser illumination causes the partial vasodilatation, alleviated has lackedthe anoxemia condition, therefore the pressure zone 1st d experiment sidecompared leans VEGFR-2 to express low (P<0.05) obviously. The weak laserhas the biology to stimulate the effect, strengthens each kind of enzymeactiveness, affects the cell membrane to pass the penetrability. After the toothweek organization absorbs the laser energy to transform the energy of light asorganization's internal energy. Because the weak laser has the biology tostimulate the effect and the cumulative effect, along with the exposure doseincrease, pressure zone 3rd d～5d the VEGFR-2 expression advances. Tensionarea 3rd d～7 the d experiment side compares leans the VEGFR-2 expressionto elevate obviously. The weak laser illumination can promote to beunbalanced effectively around the tooth to organize the VEGFR-2 expression.The weak laser partial illumination method has applied in is being unbalancedclinically, took one kind of acceleration is being unbalanced the auxiliarymethod which the tooth moves hopeful further to promote.May obtain as soon as through this experiment draws a conclusion:1. normal rabbits teeth week organization has the VEGFR-2 expression...
|
PDF Full Text Request