| Object Some chemistry materials of artificial synthesis in environment have the estrogen action. These chemistry materials can interference normal endocrine action of the birds, crawl along indoles and the mammalia wildlife. Bisphenol A is the extensive existent environmental estrogen and be used as a kind of chemical engineering raw material mainly being used for the production of macromolecule materials such as the polycarbonate, epoxy resin and unsaturated polyester resin. At the medicine aspect, it is a kind of drug that kills the fundus. Moreover, Bisphenol A is used in the production of epoxy resins and polycarbonate plastics. These plastics are used in many foods and drink packaging applications, whilst the resins are commonly used as lacquers to coat metal products such as food cans, bottle tops and water supply pipes. In 70's in 20 centuries, The people still has no right understanding to the toxicity of the Bisphenol A. Today, Bisphenol A even still was used for the raw material of the milk bottle. Along with the progress of the ages, the people had understanding to the toxicity of the Bisphenol A. In 1980, people are gradually explicit the toxicity of Bisphenol A to fish and infuse the larger enthusiasm into the research of its toxicity to deluxe animal. Bisphenol A can cause the outbreak rates of leukemia and lymphadenoma of the big rat to go up. But still have no the report that Bisphenol A can cause injury of the liver and the immune system. This research observation the changes of liver and spleen some Bio-chemical index signs when mouse be treated different dosage Bisphenol A and study the effects of Bisphenol A to the organs outside of reproduction system and investigate the injury paths. Methods To choose Kunming mouse that the animal department of our school provide, total 160, each half of male and female, body weight was 20±2 g.80 mice were divided into 8 groups random (one normal group and seven experiment groups), 10 per group. To inject the BPA (20 μmol/kgbw) in the mice belly cavity of the Experiment groups. To inject the oil in the mice belly cavity of the normal group. After the injection 30 min, 4 h, 12 h, 24 h, 36 h, 48 h, 72 h, to weigh and kill the mice, separating the liver, spleen immediately, clean the fat of surface weighs directly, computing the ratio of organ. Another 80 mice were divided into 4 groups random, 20 per group. To inject the BPA (4 μmol/kgwb, 40 μmol/kgwb, 80 μmol/kgwb) in the mice belly cavity of the Experiment groups. Each group according to the 1ml/100 g to injected BPA everyday once. The normal group was injected oil everyday. After injecting one week continuously, the half animals were sentenced to death, the surplus half animal sentences to death after injecting BPA two weeks continuously. To take the blood form the eyeball of animal, measuring the total albumen, content of TNF-αand the activity of ALT, AST. To take the liver, putting to into 10% neutral formaldethde solution affix immediately, the ethanol gradient anhydro -, the paraffin wax embedding, interrupted even cut into 5-10 slices, cut into slices 5 μm of thickness, the haematoxylin-eosin (HE) dyeing, observed appearance variety with light microscope. To take the spleen asepsis, putting into even basin with the 5 ml Hank's, comminution, lautering, leave loop(1000 r/min,5 minutes), adjust the cell concentration, join cell suspension in 96 aperture cultivation boards, the add to ConA, hatch 48 hours, then in develop cell the each aperture add to MTT, continue to develop 4 hours. To measure the value of absorb light in the 570 nm by enzyme mark instrument, compute the stimulus index number ( SI); to put the splenocyte (2 ×106/ ml) through nylon nets into the cold ethanol, refrigerator internal fixation 18 hours at 4℃. Dyeing, FCM assaying cell cycle. Result After injected BPA 0.5 h, the ratio of male mice liver has not the obvious descent, the ratio was obvious low in matched normal group after 4h, at 12 h decline to the lowest point, recover gradually henceforth, go to the normal level at the 48 h; The ratio of male mice spleen went up, got to the apogee at 4 h, then start to descend, recover the normal level at the 12 h, at the 24 h and 36 h the ratio has no obvious differentiation in matched normal group, decliningto the lowest point at 48 h, obvious low in matched control, recover gradually henceforth, went to the normal level at 72 h. The ratio of female mice liver has the obvious descent, the ratio was obvious low in matched normal group after 4h, 12h, 24h, 36h; recover gradually henceforth, went to the normal level at 48 h. The ratio of female mice spleen went up, is obvious high in matched control at 12 h and 24 h, then start to descend, decline to the lowest point at 48h, obvious low in matched control, recover gradually henceforth, went to the normal level at 72 h. After injected BPA one week, the serum total protein of each experiment group were obvious low in matched control (p<0.05), did not show the obvious difference each other. Serum ALT content of each experiment group was obvious low in matched control (p<0.05), the medium and high dosage groups were obvious low in matched low dosage group (p<0.05), the medium and high dosage groups have no obvious difference. Serum AST content of each experiment group was obvious low in control (p<0.05), the medium dosage group was obvious low in matched low dosage group (p<0.05), the low and high dosage groups have no obvious difference. Serum TNF-αcontent of each experiment medium dosage and high dosage group was obvious high in control (p<0.05), the medium dosage group was obvious low in matched low dosage group (p<0.05), the low and high dosage groups have no obvious difference. After injected BPA two weeks, the serum total protein of each experiment group was obvious high in matched control (p<0.05), the medium and high dosage group did not show the obvious difference each other. Serum ALT content of each experiment group was obvious low in matched control (p<0.05), the medium and low dosage groups have no obvious difference. Serum AST content of medium and high dosage group was obvious high in matched control (p<0.05), the medium dosage group was obvious high in matched high dosage group (p<0.05). Serum TNF-αcontent of medium dosage and high dosage group was obvious high in control (p<0.05), the medium dosage and high dosage was obvious high in matched low dosage group (p<0.05). After injected BPA one week, the G0/G1 period percentage of medium... |
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