The Cooperatively Protective Effect Of L-arginine And Branched-Chain Amino Acids On Relative Ischemia/reperfusion Injury In Rat Myocardium

ABM: The oxygen demand of myocardium increases acutely during excessive exercise and labor. Consequently blood flow in coronary arteries is relatively defcient, which results in relative ischemia/reperfusion injury. It is pathophysiologically analogous with ischemia/reperfusion injury. Both L-Arg and BCAA have much biological functions in many ways. Research results showed that they both have diffierently protective effect on relative ischemia/reperfusion injury in rat myocardium by different means. So far whether they both have cooperativly protective effect on ischemia/ reperfusion injury in myocardium has not been reported yet. Therefore this study was conducted to explore the cooperatively protective effect of L-Arg and BCAA on ischemia/reperfusion injury in rat myocardium and to illustrate its mechanism .METHODS: Model of Langendorff was used in isolated rat heart perfusion. Relative ischemia protocol: The isolated rat hearts were stimulated through the weak current for 30 minutes. 40 male Wistar rats were randomly divided into 5 groups(n=8):(1)Control group(CN):perfusion 75 min; (2) Ischemia/reperfusion injury group(IRI); (3) L-arginine (2.5mmol/L)group(L-Arg);(4)Branched-chain amino acids(Leu: 0.492g/L; Ile: 0.0275g/L; Val: 0.0476g/L) group(BCAA);(5)L-arginine(2.5mmol/L)+ Branched-chain amino acids(Leu: 0.492g/L; He: 0.0275g/L; Val: 0.0476g/L) group(L-Arg +BCAA).From (2) to (5) group the same perfusion pattern was conducted in the experiment: relative ischemia 30 min ,reperfusion 30 min. The change of cardiac function was recorded at baseline...