| Objective: To observe whether Human cytomegalovirus (HCMV) pp65 DNA vaccine can block the infection of fetal mice from their maternal mice or not thro u gh the vaccine we had researched by ourselves . It is significant for preventing and curing HCMV infection of couple of gravida and reproductive life , in the same time, for obviating disability children of nerve and raising pop μ lation predisposition . Method: 24 female healthy Balb/C 6-8 weeks old mice were randomly divided into two groups(A^ B), each group included 12 mice. In group A: 200 μg pp65 DNA vaccine was injected into Balb/C mice via muse μ lus quadriceps fexoris of left side hind legs, The mice were immunized three times at 0, 2, 4 week; In group B: 0.3ml normal saline was injected into Balb/C mice via musc μ lus quadriceps fexoris of left side hind legs. The third injection before immunization, each female mice matched with male mice. From the third day after cyesis, each female mice had conceived were injected by HCMV AD 169 strain solution of 5.0 log TCID50, and the serum were drew from orbit vein on 17th day. To assay the level of lgG, T lymphocyte and cytokine in serum with the method of ELESA, flow cytometey and radio-immunity technique and compare the difference between the two groups. To observe and compare the birth state and the mortality rate of the young mice after one week. Result: There was remarkable difference in the level of lgG, T lymphocyte and cytokine in serum between two groups, and the immunity level of group A was higher than that of group B; There was remarkable difference in fetal death rate and the mortality rate of young mice after a week, group A was lower than group B. Conclusion: HCMV pp65 DNA vaccine can cause the humoral and cell μ lar immunity; HCMV pp65 DNA vaccine can block the intrauterine emission of the mice thro μ gh reinforcing the immunity level , especially the cell μ lar immunity of the mice. |
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