The Research On Mechanism Of Costimulatory Blockade Down-regulating Immune Responses Following Small Bowel Transplantation In Mouses

Objective In order to explore the role of IDO expression in the effect ofdown-regulating immune response induced by costimulatory blockade followingsmall bowel transplantation in mouse and interpret the underlying mechanisms.Ascostimulatory blockade using cytotoxic T lymphocyte-associated antigen4 immunoglobulin (CTLA4-Ig) efficiently down-regulates immune responsesafter transplantation in animal models and is currently used in autoimmune andtransplantation clinical trials, but the precise cellular and molecular mechanismsinvolved remain unclear. Method Heterotopic small bowel transplantation(SBT) models were established with inbred strain BALB/c and C57BL/6 bymicrosurgical technique,including the isograft group (BALB/c→ BALB/c) theallograft group(C57BL/6→BALB/c)the allograft group treated with cyclosporineA (C57BL/6→BALB/c,CsA20mg-Kg-l-d) and the treated group of CTLA4-Ig...