| Objective: To study immune tolerance induced by CTLA4-Ig afterinstrasplenic hepatocyte transplantation for treatment of D-galactosamineinduced acute liver failure in rat.Methods: The mold of acute chemical liver failure: SD rats were injected 10%D-galactosamine 1.0g/Kg into abdominal cavity.Livers were perfusing withthree different liquids, firstly with D-Hanks including EDTA, secondly withD-Hanks, finally with collagenase type Ⅳ. Consistency of hepatocytes was 4×10~7/ml. Some of hepatocytes were transplanted through spleens within twohours. At twenty-four hours after injected with D-galactosamine, the twenty-fourrats were received allogeneic hepatocytes through spleens, and were randomlydivided into two groups. One group was received CTLA4-Ig by intraperitonealinjection. The concentration of IL-2 and TNF, liver function and histologicywere observed at the 1st,3rd,5th, 7th day after operation.Results: IL-2 of the experimental group was significantly decreased afteroperation, P<0.05. TNF had no difference between two groups after operation,P>0.05. The liver function of experimental group (ALT, TBiL) standinglyreduced, P<0.05. Histological changes: At the 7th day after operation, there weresome hepatocytes in the experimental group's spleens. But in the control group'sspleens, the changes were characterized by severe lymphocyte infiltration. Andthere were not hepatocytes in them.Conclusions: CTLA4-Ig can induce rat allogeneic hepatocytes intrasplenictransplantation immune tolerance. It may improve the liver function of acuteliver failure rats.
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