The Effect Of Alpha Lipoic Acid On The Expression Of Glutamine: Fructose-6-Phosphate Amidotransferase-1 In Diabetic Mouse Skeletal Muscle

Objective:To learn the effect of alpha-lipoic acid on the expression of glutamine: fructose-6-phosphate amidotransferase 1, which is the rate-limiting enzyme of hexosamine biosynthesis pathway, in the diabetic mouse hind-limb skeletal muscle, and provide the theory basis for applying the antioxidant alpha-lipoic acid in preventing and treating diabetes. Methods:1. Diabetes was induced with a single intravenous injection of alloxan into KM mice.2. Diabetic mice were divided into four groups at random. The placebo (30mg/kg ip) or different doses alpha-lipoic acid (15,30,60mg/kg ip) was administered daily to these mice for fourteen days.3. Malondialdenhyde levels serve as a marker for lipid peroxidation. These were measured using thiobarbituric acid in the serum and the skeletal muscle among normal and diabetic mice.4. The expression of glutamine: fructose-6-phosphate amidotransfer -ase 1 gene was analyzed by semi-quantitative reverse transcrip -tion polymerase chain reaction.Result:1. The incidence of diabetes induced by alloxan was 69.2%.2. The placebo group's MDA content in the serum and the skeletal muscle was the highest among groups (P<0.05). In the serum, the second was the low dosage group (P<0.05), and there was no significantly different among normal, medium dosage, and high dosage (P>0.05). The skeletal muscle's MDA content reduced with the increase of alpha-lipoic acid among diabetic mice (P<0.05), but between high dosage group and normal group there was no obviously different (P>0.05).3. In the skeletal, the placebo group's glutamine: fructose-6 -phosphate amidotransferase 1 mRNA level is as more as the low dosage group's (P>0.05), however, it's mean was higher than the low dosage group's. The two groups were higher than the medium and high dosage groups (P<0.05), and between the later two groups there was no significantly...