A Study For Electrochemotherapy And Permittivity Measurement Of Micro Biological Samples

The cell membrane is the main barrier for the macromolecules entering into a cell. Electroporation is a biophysical phenomenon referring to the formation of transient reversible micro-pores on the phospholipid bilayer of cell membrane under the transient electric field. Because of the increase of membrane's permeability, electroporation is widely used in many aspects such as DNA transfection, protein introduction cell electrofusion et al.In this paper it was used to the treatment of cell in-vitro and malignant tumour. The tumor was injected the chemotherapy medicine followed by the radiation of electromagnetic pulses, which is know as Electrochemotherapy(ECT). And it was verified that ECT could greatly improve the treatment effect of tumour.The permittivity of living organisms is an important physical parameter reflecting the polarization of the medium under the field. The change and difference of dielectric properties of tissue and organs help to diagnose and analyze the abnormal tissue, which has certain application prospects in the clinical diagnosis and treatment of tumour and other diseases. Biological samples are very limited usually, and resonant-cavity perturbational method especially fit for the measurement of micro amount samples settled with the limitation of sampling.In this paper the simplified equations for computing the complex permittivity is gained by detailed analyses of the resonant-cavity perturbational theory. Given the biological materials' characteristic of high moisture, high permittivity and high loss, some methods were taken to improve the system's precision. Such as enlarging the volume of resonant cavity, reducing the volume of samples, determining volume of samples more accurately etc. Through the measurement of water and NaCl solution at different concentration, it was proved the precision of the system is relatively high. For the first time, the complex permittivities of tumour cell sap and blood of rats undergone ECT were measured, which could be regarded as a reference in the study of bioelectromagnetic effect and a preparative work in clinical examination of the characteristic of tissues.