| OBJECTIVES: We created a novel method by implantation of autologous marrow mesenchymal cells (MSCs) through cardia catheter after successfully creating the model of myocardial infarction in swine by embolization of the left coronary artery using a gelatin sponge through cardiac catheter. This study was to investigate whether the new therapy can result in angiogenesis and improvement of cardia function in myocardial infarct model of swine.METHODS: 20 milliliter bone marrow was aspirated from the postero- superior iliac spine of Taihu Meishan swine. The mesenchymal stem cells (MSCs) were cultured according to the methods of Wakitai's. After myocardial infaraction, ten swines were equally divided into experimental group (B group ) and control group (A group) randomly. The same volume of the MSCs or culture medium was injected into the infracted myocardium through cardiac catheter. Four weeks after implantation, the swines were anesthesized for echocardiogaphic. We also tested the VEGF and ET level in serum to explore the mechamism of improvement of cardiac function after cellar transplantation. In the end, swines' heart were excised to make pathological sections, basic changes were examined by H.E. stain. Vessel number was counted in experimental group and control group byimmuno-histochenistry study with CD31 monoclonal antibody.RESULTS: 1) Left ventricular function, including ejection fraction (EF), left ventricular end-systolic dimension (LVESD), left ventricular end-diastolic dimension (LVEDD), ets. was significantly improved in B group when compared with A group. 2) Immunohisto-chemistry study showed that vessels stained with CD31 mAb was much more than control group. 3) Serum levels of VEGF in B group were significantly elevated whereas the levels of ET in B group were decreased step by step.CONCLUSIONS: 1) The model of myocardial infarction is reliable, reproducible, and similar to the human condition, amenable to investigate other investigation. 2) The method we used for MSCs isolation and culture was feasible. 3) The therapy of implantation of autologous mesenchymal stem cells through cardiac catheter was effective, feasible and safe in ischemic heart disease. 3) Implantation of MSCs seems to improve the cell viability and blood perfusion in the infracted area, indicating that MSCs induce angiogenesis. 4) MSCs, after being implanted into infracted myocardium, showed the ability of secreting VEGF with subsequent angiogenesis, which can lead ET levels' reduction.
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