To Explore The Significances And Expressions Of P-selectin And Its Ligands In Esophageal Squamous Cell Carcinoma By Using Tissue Microarray Technique

Objective: To investigate the pathological relationship between the expressions of P-selectin and its ligands in esophageal squamous cell carcinoma and clinical significance.Methods: Pathological specimens from 86 esophageal squamous cell carcinomas,86 corresponding paracancerous lymph node tissues and 10 esophageal normal tissues were examined for P-selectin and its ligands by tissue microarray technique and immunohistochemistry.Results: (l)The positive expression rates of P-selectin in esophageal squamous cell carcinoma, paracancerous lymph node tissues and esophageal normal tissues were 65.1%,69.8%,0.0%,respectively. The positive expression rates of P-selectin in esophageal squamous cell carcinoma group and paracancerous lymph node tissues group were obviously higher than that in esophageal normal tissues group, and the difference was significant enough to be of statistical value(P<0.01);(2)No statistical difference of P-selectin expression rates was found among of the groups which were divided by sex, age, tumor location,tumor differentiation and the length of tumor, respectively (PX).05);(3)The positive expression rates of P-selectin were 75.0%,36.4% in group with lymph node metastasis and group without lymph node metastasis respectively, and the difference was significant enough to be of statistical value(P<0.01);(4)The positive expression rates of P-selectin were 92.0%, 54.1% in group with tumor invasion of stage T3+T4 and group with tumor invasion of stage T1+T2 respectively, and the difference was significant enough to be of statistical value (P<0.01);(5)The positive P-selectin expression rates of stage I ,II,III,IVwere 37.5%,55.6%,75.8%,81.8%, respectively. The positive P-selectin expression rate of stage III+IV was obviously higher than that of stage I + II, and the difference was significant enough to be of statistical value(P<0.05);(6)The positive expression rates of sialyl Lewis a in esophageal squamous cell carcinoma, paracancerous lymph node tissues and esophageal normal tissues were76.7%,74.4%,10.0%,respectively. The positive expression rates of sialyl Lewis a in esophageal squamous cell carcinomagroup and paracancerous lymph node tissues were obviously higher than that in esophageal normal tissues group, and the difference was significant enough to be of statistical value(P<0.01);(7)The positive expression rates of sialyl Lewis a in esophageal squamous cell carcinoma of grade I , II, III were20.0%,78.3%,93.3%, respectively, and the difference was significant enough to be of statistical value(P<0.01);(8)The positive expression rates of sialyl Lewis a were 92.0%,70.5% in group with tumor invasion of stage T3+T4 and group with tumor invasion of stage T1+T2 respectively, and the difference was significant enough to be of statistical value (P<0.05);(9)The positive expression rates of sialyl Lewis a were 89.1%,40.9% in group with lymph node metastasis and group without lymph node metastasis respectively, and the difference was significant enough to be of statistical value(P<0.01);(10)The positive sialyl Lewis a expression rates of stage I, II ,III IV were 25.0%,70.6%,90.9%,90.9%, respectively. The positive sialyl Lewis a expression rate of stage III+IV was obviously higher than that of stage I + II, and the difference was significant enough to be of statistical value(P<0.01);(11)The positive expression rates of sialyl Lewis X in esophageal squamous cell carcinoma, paracancerous lymph node tissues and esophageal normal tissues were 14.0%,11.6%,10.0%,respectively. No statistical difference of sialyl Lewis X expression was found among of them (P>0.05).Conclusion: P-selectin and sialyl Lewis a may play important roles in the development and progression of esophageal squamous cell carcinoma. Expression of P-selectin and sialyl Lewis a is related to the clinical biological behavior of esophageal squamous cell carcinoma. The overexpression of P-selectin and sialyl Lewis a predict high possibility of invasion and metastasis of the esophageal squamous cell carcinoma. It is practical to utilize tissue microarray technique which is a rapid, economic and accurate to screen the clinical tissue specimens on a large scale.