Apoptosis Induction Of Prostate Cancer Cells With RNA Interference Inhibiting Survivin Expression

Objective To construct the plasmids encoding two survivin short hairpin RNA (shRNA) and investigate its effects on Hormone -Refractory Prostate Cancer cells line PC-3.Methods Two shRNA of survivin were designed and synthesized respectively, then both of them were inserted into plasmids. The recombinant plasmids were confirmed by sequencing and agarose gel electrophoresis after restriction digestion. After transfection of it to PC-3 cells using Metafectene for 48 hours, the result was detected by RT-PCR, Western blotting and flow cytometer . PC-3 cells grow activity was also studied.Results The recombinant plasmids were constructed and the aim sequence was obtained. Results Hairpin RNA could reduce the expression of survivin mRNA and survivin protein significantly. Meanwhile, the cells growth was inhibited and the apoptosis of PC-3 cells increased dramaticly.Conclusion After transfection, the recombinant plasmids could cut down the expression of survivin mRNA and survivin protein in PC-3 cells. The result was significantly lower than that in controls.Suppression of survivin mRNA and survivin protein could inhibit the proliferation of PC-3 cells and increase apoptosis of PC-3 cells significantly.