Survivin ShRNA Construction Of Adenovirus Vector And Apoptosis Induction Of Prostate Cancer Cells

Objective:To construct adenovirus vector encoding two survivin short hairpin RNA (shRNA) and investigate its effects on Hormone -Refractory Prostate Cancer cells line PC-3.Methods:Two shRNA of survivin were designed and synthesized respectively,then both of them were inserted into adenovirus DNA. The recombinant adenovirus vector were confirmed by sequencing and agarose gel electrophoresis after restriction digestion. This recombinant construct was then linearlized and transfected into HEK 293 cell line to generate recombinant adenovirus.After transfection of it to PC-3 cells for 48 hours, the result was detected by RT-PCR,Western blotting and flow cytometer .Results:The recombinant adenovirus vector were constructed and the aim sequence was obtained. Results Hairpin RNA could reduce the expression of survivin mRNA and survivin protein significantly. Meanwhile, the cells growth was inhibited and the apoptosis of PC-3 cells increased obviously.Conclusion:After powerful transfection,the recombinant adenovirus could cut down the expression of survivin mRNA in PC-3 cells. The result was significantly lower than that in controls. Suppression of survivin mRNA...