Inhibitory Effects Of Panaxynol On The Proliferation And Migration Of Vascular Smooth Muscle Cells Induced By PDGF-BB

AIM:The inhibitory effects of panaxynol(PAN) on the proliferation and migration of vascular smooth muscle cells(VSMCs) induced by PDGF-BB were investigated. METHODS:3～8 passages of primary cultured rat aortic smooth muscle cells (RASMCs) were used. RASMCs were grouped as follows: ①control(10%FBS DMEM); ②PDGF-BB(20ng/ml); ③1, 3, 9μmol·L-1PAN(contained PDGF-BB). The effects of different concentrations of PAN on the proliferation and migration of RASMCs were evaluated by MTT and cell scrape tests. The influences of PAN on the total protein concentration were measured with coomassie brilliant blue and the effects of PAN on the expression of ERK1/2 were studied by western blotting analysis. RESULTS : MTT tests showed that PDGF-BB could increase OD values significantly compared with control. 1, 3, 9μmol·L^-1PAN treatment decreased OD values obviously and inhibitory rates were 12.9%(p<0.05), 23.1%(p<0.05) and 40%(p<0.01) respectively. The results suggested that PAN could inhibit RASMCs hyperplasia induced by PDGF-BB concentration-dependently. Cell scrape tests showed that PDGF-BB could increase the distance of migration in RASMCs compared with control(p<0.01). 1, 3, 9μmol·L^-1 PAN counteracted the action of PDGF-BB, inhibitory rates of migration were 8.1%(p<0.05), 18.3%(p<0.05) and 67.3%(p<0.01) respectively, which indicated that PAN could inhibit the RASMCs migration induced by PDGF-BB. In addition, results showed that 1, 3, 9μmol·L-1PAN decreased total protein comcentration significantly(P<0.05 or P<0.01). Moreover, 1, 3, 9μmol·L-1PAN could decrease the expression of ERK1/2 in RASMCs. The above results indicated that downregulated expression of ERK1/2 might be responsible for the inhibitory effect of PAN on PDGF-BB-induced by hyperplasia and migration. CONCLUSIONS: PAN could inhibit the RASMCs proliferation and migration induced by PDGF-BB, and decrease of ERK1/2 expression might be one of its mechamisms. Our study may provide insight into the new drug development strategy for preventing and treating such cardiovascular diseases as hypertension, atherosclerosis, restenosis etc.