Preparation And Targetibility Of Antitumor Immunoliposomes Entrapping ADM Bearing Anti-VEGF Ab

Objective:To prepare immunoliposomes,and examine the peculiar cytotoxicity of immunoliposomes on targeting cells. At the same time, we study the effect of immunoliposomes act on targeting cells with different time and diffrernt concentration.Methods: A driamycin liposomes (ADMLs) were prepared by a pH-gradient method and determining the entrapping rate of ADM in liposomes by Visible and Ultraviolet Spectroscopy at 232 nm after the free driamycin molecules was separated from ADMLs on a sephadex G-50 minicolumn. Then we prepared immunoliposomes by conjugating anti-VEGF monoclonal antibodies through glutara-ldehyde to liposomes. After the monoclonal antibodies was seperated from ADMILs by a sephadex G-200 minicolumn , the peculiar cytotoxicity of immunoliposomes on T-24 was studied, at the same time we used the normal peripheral lymphocytes as control-group cells.Results: The entrapping rate of ADM in liposomes is 98.1 percent. Theliposomes were linked with anti-VEGF monoclonal antibody.Using T-24 as targeting cells and normal peripheral lymphocytes as control-group cells, we examined the peculiar cytotoxicity of immunoliposomes on targeting cells and control-group cells, respectively. The death rate of targeting cells after action for 96 hours is 60 percent , whereas that ofcontrol-group cells is only 8 percent ,from which we know that the immunoliposomes can kill their targeting cells peculiarly.Conclusions: The entrapping rate of liposomes is comparatively high. And the immunoliposomes bearing monoclonal antibody prepared from liposomes are peculiar to their targeting cells. The death rate of targeting cells after action for 96 hours is 60 percent , while the control-group cells is only 8 percent...